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Anti-HSPA2 Polyclonal Antibody

Catalog #:   PHF04101 Specific References (20) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P54652
Overview

Catalog No.

PHF04101

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human HSPA2 (Ser2-Glu98).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

HSPA2, Heat shock-related 70 kDa protein 2, Heat shock 70 kDa protein 2

Purification

Purified by antigen affinity column.

Accession

P54652

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with HSPA2 antibody (PHF04101) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 13 kDa
    Observed MW: 13 kDa
References

Characterised intron retention profiles in muscle tissue of idiopathic inflammatory myopathy subtypes., PMID:38302260

Targeted Analysis of HSP70 Isoforms in Human Spermatozoa in the Context of Capacitation and Motility., PMID:35742939

Immunofluorescence and High-Resolution Microscopy Reveal New Insights in Human Globozoospermia., PMID:35163651

Human sperm chaperone HSPA2 distribution during in vitro capacitation., PMID:33246276

Various Anti-HSPA2 Antibodies Yield Different Results in Studies on Cancer-Related Functions of Heat Shock Protein A2., PMID:32560263

Evaluating the Potential of Three Sperm Surface Antigens as Egg-adhesion Biomarkers for Human Sperm Selection., PMID:30746335

Assessing the potential of HSPA2 and ADAM2 as two biomarkers for human sperm selection., PMID:30463455

Dataset on gene expression profiling of multiple murine hair follicle populations., PMID:27672671

Knockdown of polycomb-group RING finger 6 modulates mouse male germ cell differentiation in vitro., PMID:25591775

Exon B of human surfactant protein A2 mRNA, alone or within its surrounding sequences, interacts with 14-3-3; role of cis-elements and secondary structure., PMID:23525782

Evaluation of HSPA2 in fertile and infertile individuals., PMID:22670834

Heat shock proteins on the human sperm surface., PMID:19962198

The HspA2 protein localizes in nucleoli and centrosomes of heat shocked cancer cells., PMID:18452162

Cellular maturity and apoptosis in human sperm: creatine kinase, caspase-3 and Bcl-XL levels in mature and diminished maturity sperm., PMID:15044602

Potential role of nuclear factor kappaB and reactive oxygen species in cAMP and cytokine regulation of surfactant protein-A gene expression in lung type II cells., PMID:12040027

Decreased expression of the heat shock protein hsp70-2 is associated with the pathogenesis of male infertility., PMID:11730740

Expression of HSP70-2 gene during germ cell apoptosis in rat unilateral cryptorchid testes., PMID:11297064

Isolation and identification of sperm membrane antigens recognized by antisperm antibodies, and their possible role in immunological infertility disease., PMID:11160836

Putative creatine kinase M-isoform in human sperm is identifiedas the 70-kilodalton heat shock protein HspA2., PMID:10952940

Specific expression of heat shock protein HspA2 in human male germ cells., PMID:10587366

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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Distributor list

For research use only. Not for human or drug use.

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Anti-HSPA2 Polyclonal Antibody [PHF04101]
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