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Anti-Human CHAT Polyclonal Antibody

Catalog #:   PHD78101 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P28329
Overview

Catalog No.

PHD78101

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CHAT (Met1-Pro630).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Choline acetylase, CHOACTase, Choline O-acetyltransferase, ChAT, CHAT

Purification

Purified by antigen affinity column.

Accession

P28329

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with CHAT antibody (PHD78101) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 73 kDa
    Observed MW: 73 kDa
References

Accuracy and Reproducibility of ChatGPT Responses to Breast Cancer Tumor Board Patients., PMID:40466030

Harnessing synaptic vesicle release and recycling with antibody shuttle for targeted delivery of therapeutics to neurons., PMID:40454418

Neuroimaging and immunofluorescence of the Pseudopus apodus brain: unraveling its structural complexity., PMID:40434439

Immunological response and implications of Ad26.COV2. S (Janssen COVID-19 vaccine) vaccine in diabetic patients: a prospective cohort study in Ethiopia., PMID:40406137

Unique characteristics of autoantibodies targeting MET in patients with breast and lung cancer., PMID:40401526

Discovery of the Aβ receptor that controls the voltage-gated sodium channel activity: unraveling mechanisms underlying neuronal hyperexcitability., PMID:40298589

Targeting mitochondrial dynamics emerges as an effective strategy of cardioprotection against trastuzumab-induced mitochondrial functional aberrations and cardiotoxicity in rats., PMID:40288556

B cells modulate lung antiviral inflammatory responses via the neurotransmitter acetylcholine., PMID:40263611

Toxoplasmosis infection and schizophrenia: Elevated IgM reactivity index as a predictor of symptom worsening in chronic schizophrenia., PMID:40088603

Interest in Rh testing and Rh immunoglobulin treatment among patients obtaining telehealth medication abortion., PMID:40021111

Effect of Anti-TNF Monoclonal Antibody on Enteric Neurons and Enteric Glial Cells in Experimental Colitis., PMID:39946069

A Statewide Telemedicine Referral System for Regional Monoclonal Antibody Infusion Centers., PMID:39882985

Regulatory T cell expansion prevents retinal degeneration in type 2 diabetes., PMID:39716335

Safety and immunogenicity of an inactivated recombinant Newcastle disease virus vaccine expressing SARS-CoV-2 spike: A randomised, comparator-controlled, phase 2 trial., PMID:39615342

Experimental study of cBMMSC based on nanosilver hydrogel nerve conduit for repairing spinal cord injury., PMID:39588835

CD2AP is Co-Expressed with Tropomyosin-Related Kinase A and Ras-Related Protein Rab-5A in Cholinergic Neurons of the Murine Basal Forebrain., PMID:39211110

Tyrosine Protein Kinase SYK-Related Gene Signature in Baseline Immune Cells Associated with Adjuvant Immunotherapy-Induced Immune-Related Adverse Events in Melanoma., PMID:39115425

Neocortical cholinergic pathology after neonatal brain injury is increased by Alzheimer's disease-related genes in mice., PMID:39111704

The role of the Ventral Nucleus of the Trapezoid Body in the auditory prepulse inhibition of the acoustic startle reflex., PMID:38972084

ChatGPT as a New Tool to Select a Biological for Chronic Rhino Sinusitis with Polyps, "Caution Advised" or "Distant Reality"?, PMID:38929784

Is generative pre-trained transformer artificial intelligence (Chat-GPT) a reliable tool for guidelines synthesis? A preliminary evaluation for biologic CRSwNP therapy., PMID:38329526

Circulating extracellular choline acetyltransferase regulates inflammation., PMID:38011942

Distribution, quantification, and characterization of substance P enteric neurons in the submucosal and myenteric plexuses of the porcine colon., PMID:37982872

Vagus nerve stimulation and acetylcholinesterase inhibitor donepezil provide cardioprotection against trastuzumab-induced cardiotoxicity in rats by attenuating mitochondrial dysfunction., PMID:37816466

Can Scoliosis Help the Early Diagnosis of Congenital Myasthenic Syndrome?, PMID:37766777

New insight on the enteric cholinergic innervation of the pig colon by central and peripheral nervous systems: reduction by repeated loperamide administration., PMID:37650102

A Semi-Three-Dimensional Bioprinted Neurocardiac System for Tissue Engineering of a Cardiac Autonomic Nervous System Model., PMID:37508861

Acetylcholine receptor agonists effectively attenuated multiple program cell death pathways and improved left ventricular function in trastuzumab-induced cardiotoxicity in rats., PMID:37482212

Expanded insights into the neural component of the activity-based anorexia animal model - morphological changes in the enteric nervous system and altered pain perception., PMID:37406277

Identification of Lineage-specific Transcriptional Factor-defined Molecular Subtypes in Small Cell Bladder Cancer., PMID:37380560

Germline immunomodulatory expression quantitative trait loci (ieQTLs) associated with immune-related toxicity from checkpoint inhibition., PMID:37301715

Changes in Starburst Amacrine Cells in Mice with Diabetic Retinopathy., PMID:37258479

Extract of Aster koraiensis Nakai Leaf Ameliorates Memory Dysfunction via Anti-inflammatory Action., PMID:36982837

Morphology and immunohistochemical characteristics of the otic ganglion in the chinchilla (Chinchilla laniger Molina)., PMID:36692115

Evaluation of eGFP expression in the ChAT-eGFP transgenic mouse brain., PMID:36650430

Anatomic and histomorphometric study of the nerve to the vastus lateralis in cadaver for its clinical application in facial reanimation., PMID:36645336

Nitrergic and Purinergic Nerves in the Small Intestinal Myenteric Plexus and Circular Muscle of Mice and Guinea Pigs., PMID:36587144

Connections between postparotid terminal branches of the facial nerve: An immunohistochemistry study., PMID:36271803

Anatomical study of the adductor canal: three-dimensional micro-computed tomography, histological, and immunofluorescence findings relevant to neural blockade., PMID:36245345

Human Pulmonary Vein Myocardial Sleeve Autonomic Neural Density and Cardiovascular Mortality., PMID:36154512

Assessing PfGARP-Mediated Apoptosis of Blood-Stage Plasmodium falciparum Parasites., PMID:35881381

Human OTULIN haploinsufficiency impairs cell-intrinsic immunity to staphylococcal α-toxin., PMID:35587511

Substrate recognition and cryo-EM structure of the ribosome-bound TAC toxin of Mycobacterium tuberculosis., PMID:35552387

Duodenal cholinergic tuft cell number is increased in functional dyspepsia., PMID:35388579

[Features of the use of synthetic analogues of thyroid hormones: а 2020 THESIS* questionnaire survey of members of the Belarusian Public Medical Association of Endocrinology and Metabolism]., PMID:35262294

Effect of sevoflurane preconditioning on sleep reintegration after alteration by lipopolysaccharide., PMID:35170121

Laboratory Diagnostic Capacity for Fungal Infections in Nigerian Tertiary Hospitals: A Gap Analysis Survey., PMID:34919363

Immunohistochemical Expression of Choline Acetyltransferase and Catecholamine-Synthesizing Enzymes in Head-and-Neck and Thoracoabdominal Paragangliomas and Pheochromocytomas., PMID:34743284

Distribution and neurochemical characteristic of the cardiac nerve structures in the heart of chinchilla (Chinchilla laniger Molina)., PMID:34581422

Peripheral Nerve Impairment in a Mouse Model of Alzheimer's Disease., PMID:34573265

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-Human CHAT Polyclonal Antibody [PHD78101]
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