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Anti-WTAP Polyclonal Antibody

Catalog #:   PHG96901 Specific References (16) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: Q15007
Overview

Catalog No.

PHG96901

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human WTAP (Met1-Asp150).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Wilms tumor 1-associating protein, Pre-mRNA-splicing regulator WTAP, hFL(2)D, WT1-associated protein, Female-lethal(2)D homolog, KIAA0105, WTAP

Purification

Purified by antigen affinity column.

Accession

Q15007

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with WTAP antibody (PHG96901) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 20 kDa
    Observed MW: 20 kDa
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with wtap antibody (PHG96901) at 1 μg/ml.

    Lane 1: Hone1

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 44 kDa
    Observed MW: 50 kDa
  • Immunohistochemical
    Immunohistochemical analysis of rat small intestine stained for wtap with PHG96901.
References

Astragalus polysaccharide alleviates IL-13-induced oxidative stress injury in nasal epithelial cells by inhibiting WTAP-mediated FBXW7 m6A modification., PMID:38957784

Prognostic value of comprehensive typing based on m6A and gene cluster in TNBC., PMID:36109402

Stabilization of UCA1 by N6-methyladenosine RNA methylation modification promotes colorectal cancer progression., PMID:34809621

Bone Marrow Mesenchymal Stem Cells-Derived Exosomal miR-425-5p Inhibits Acute Myeloid Leukemia Cell Proliferation, Apoptosis, Invasion and Migration by Targeting WTAP., PMID:34594112

RNA Demethylase FTO Mediated RNA m6A Modification Is Involved in Maintaining Maternal-Fetal Interface in Spontaneous Abortion., PMID:34350169

N6-Methyladenosine-Related RNA Signature Predicting the Prognosis of Ovarian Cancer., PMID:34137363

Chidamide increases the sensitivity of Non-small Cell Lung Cancer to Crizotinib by decreasing c-MET mRNA methylation., PMID:32792859

Decreased Peripheral Blood ALKBH5 Correlates with Markers of Autoimmune Response in Systemic Lupus Erythematosus., PMID:32685056

Epitranscriptomics in liver disease: Basic concepts and therapeutic potential., PMID:32330603

Interactions, localization, and phosphorylation of the m6A generating METTL3-METTL14-WTAP complex., PMID:29348140

Feasible etiology of vanishing testis regarding disturbance of testicular development: histopathological and immunohistochemical evaluation of testicular nubbins., PMID:22247958

Wilms tumor 1 expression in vascular neoplasms and vascular malformations., PMID:21697701

Evaluation of podocyte lesion in patients with diabetic nephropathy: Wilms' tumor-1 protein used as a podocyte marker., PMID:19969384

Wilms tumor gene protein 1 is associated with ovarian cancer metastasis and modulates cell invasion., PMID:18260155

Immunohistochemical expression of Wilms' tumor 1 protein in nephroblastoma., PMID:15648285

[Cellular and humoral immune responses against WT1 products overexpressed in hematopoietic malignancies]., PMID:12229118

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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Distributor list

For research use only. Not for human or drug use.

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Anti-WTAP Polyclonal Antibody [PHG96901]
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