Catalog No.
EWK27301
Expression system
Insect Cells
Species
Apis mellifera (Honeybee)
Protein length
Phe29-Val382
Predicted molecular weight
44.73 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
Q08169
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution.A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
Hyaluronidase, Hya, 3.2.1.35, Allergen Api m II, Hyaluronoglucosaminidase, Api m 2, Apis mellifera (Honeybee)
Low-dose Bee Venom as a Potential Therapeutic Agent Against Human Chronic Myeloid Leukaemia Cells., PMID:39924635
Comparative Assessment of the Allergenicity of Hyaluronidases from Polistes dominula (Pol d 2), Vespula vulgaris (Ves v 2), and Apis mellifera Venom (Api m 2)., PMID:39591253
Impact of environmental factors on Apis dorsata (giant Asian honeybee) venom in Bandarawela, Sri Lanka., PMID:39522659
Venom Component Allergen IgE Measurement in the Diagnosis and Management of Insect Sting Allergy., PMID:39097146
Sensitization against medical hyaluronidase in patients with confirmed hypersensitivity against hymenoptera species and its clinical implications., PMID:39005205
Peeking into the Stingers: A Comprehensive SWATH-MS Study of the European Hornet Vespa crabro (Linnaeus, 1758) (Hymenoptera: Vespidae) Venom Sac Extracts., PMID:38612607
Comparative study of the commonly used protein quantitation assays on different Hymenoptera venoms: A fundamental aspect of Hymenoptera venom proteome analysis., PMID:38503352
Production of a novel recombinant brown spider hyaluronidase in baculovirus-infected insect cells., PMID:33812558
A Novel Apilic Antivenom to Treat Massive, Africanized Honeybee Attacks: A Preclinical Study from the Lethality to Some Biochemical and Pharmacological Activities Neutralization., PMID:33466223
Insect hypersensitivity beyond bee and wasp venom allergy., PMID:33275648
Production of Active Recombinant Hyaluronidase Inclusion Bodies from Apis mellifera in E. coli Bl21(DE3) and characterization by FT-IR Spectroscopy., PMID:32485932
Characterization of Venom Components and Their Phylogenetic Properties in Some Aculeate Bumblebees and Wasps., PMID:31947554
Sensitization to Api m 1, Api m 2, and Api m 4 in Japanese beekeepers who had experienced systemic reactions to honeybee stings., PMID:30279045
Routine clinical utility of honeybee venom allergen components., PMID:30176296
IgE cross-reactivity of phospholipase A2 and hyaluronidase of Apis dorsata (Giant Asian Honeybee) and Apis mellifera (Western Honeybee) venom: Possible use of A. mellifera venom for diagnosis of patients allergic to A. dorsata venom., PMID:28712914
Sensitization to Api m 1, Api m 2, and Api m 4: association with safety of bee venom immunotherapy., PMID:25736542
Cloning and molecular characterization of scorpion Buthus martensi venom hyaluronidases: a novel full-length and diversiform noncoding isoforms., PMID:24973698
Hyaluronidase from the venom of the social wasp Polybia paulista (Hymenoptera, Vespidae): Cloning, structural modeling, purification, and immunological analysis., PMID:23305623
High sensitivity of CAP-FEIA rVes v 5 and rVes v 1 for diagnosis of Vespula venom allergy., PMID:22277201
In situ imaging of honeybee (Apis mellifera) venom components from aqueous and aluminum hydroxide-adsorbed venom immunotherapy preparations., PMID:22104605
Unbinding of hyaluronan accelerates the enzymatic activity of bee hyaluronidase., PMID:21840987
Hyaluronidase and hyaluronan in insect venom allergy., PMID:21597301
Identification of a novel melittin isoform from Africanized Apis mellifera venom., PMID:20472009
Africanized honey bee (Apis mellifera) venom profiling: Seasonal variation of melittin and phospholipase A(2) levels., PMID:20403370
Reassessing the role of hyaluronidase in yellow jacket venom allergy., PMID:19910026
Dissecting cross-reactivity in hymenoptera venom allergy by circumvention of alpha-1,3-core fucosylation., PMID:19896717
[Allergy and neurotoxicity induced by bee sting. Case report and literature review]., PMID:18693540
[Cross-reactivity to honeybee and wasp venom]., PMID:18265954
High-yield recombinant expression of the extremophile enzyme, bee hyaluronidase in Pichia pastoris., PMID:18024155
Characterization of the N-glycans of recombinant bee venom hyaluronidase (Api m 2) expressed in insect cells., PMID:17479607
Identification of a B-cell epitope of hyaluronidase, a major bee venom allergen, from its crystal structure in complex with a specific Fab., PMID:17374540
Towards abolition of immunogenic structures in insect cells: characterization of a honey-bee (Apis mellifera) multi-gene family reveals both an allergy-related core alpha1,3-fucosyltransferase and the first insect Lewis-histo-blood-group-related antigen-synthesizing enzyme., PMID:17029591
Structure of recombinant Ves v 2 at 2.0 Angstrom resolution: structural analysis of an allergenic hyaluronidase from wasp venom., PMID:16699186
Prevention of allergy by a recombinant multi-allergen vaccine with reduced IgE binding and preserved T cell epitopes., PMID:16206231
Sol i 1, the phospholipase allergen of imported fire ant venom., PMID:15753912
Identification by immunoblot of venom glycoproteins displaying immunoglobulin E-binding N-glycans as cross-reactive allergens in honeybee and yellow jacket venom., PMID:15005742
Individual hymenoptera venom compounds induce upregulation of the basophil activation marker ectonucleotide pyrophosphatase/phosphodiesterase 3 (CD203c) in sensitized patients., PMID:12403934
Regulation of hyaluronidase activity by alternative mRNA splicing., PMID:12084718
Antibody binding to venom carbohydrates is a frequent cause for double positivity to honeybee and yellow jacket venom in patients with stinging-insect allergy., PMID:11742287
Occupational allergy to bumblebees: allergens of Bombus terrestris., PMID:11692115
N-Glycan analysis by matrix-assisted laser desorption/ionization mass spectrometry of electrophoretically separated nonmammalian proteins: application to peanut allergen Ara h 1 and olive pollen allergen Ole e 1., PMID:10998264
Application of free-flow electrophoresis for isolation and purification of proteins and peptides., PMID:9662174
Superior biologic activity of the recombinant bee venom allergen hyaluronidase expressed in baculovirus-infected insect cells as compared with Escherichia coli., PMID:9600508
Allergens in Hymenoptera venom. XXVII: bumblebee venom allergy and allergens., PMID:8613638
Biochemical properties and study of antigenic cross-reactivity between Africanized honey bee and wasp venom., PMID:8081540
Bee venom hyaluronidase is homologous to a membrane protein of mammalian sperm., PMID:7682712
Immunoblot analysis of IgE and IgG antibodies to honey bee venom: cross sectional and sequential studies in bee sensitive subjects., PMID:1807261
Biochemical and immunochemical comparison of Africanized and European honeybee venoms., PMID:2299109
Analysis of differing patterns of cross-reactivity of honeybee and yellow jacket venom-specific IgE: use of purified venom fractions., PMID:2499550
Histamine release from peripheral blood leukocytes with purified bee venom allergens: effect of hyperimmune beekeeper plasma., PMID:2471697
For research use only. Not for human or drug use.
