Catalog No.
RVV14202
Expression system
Mammalian Cells
Species reactivity
Vaccinia
Host species
Human
Isotype
IgG1, kappa
Clonality
Monoclonal
Target
Protein A33, VACWR156, A33R
Concentration
2.42 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
P68617
Applications
ELISA, Neutralization
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
A27D7
Levels of antibodies against the monkeypox virus compared by HIV status and historical smallpox vaccinations: a serological study., PMID:38767199
Comparison of the immunogenicity and protective efficacy of ACAM2000, MVA, and vectored subunit vaccines for Mpox in rhesus macaques., PMID:38536937
Examination of the cross-reactivity between vaccinia virus Tiantan strain and monkeypox virus., PMID:37473582
Particle-specific neutralizing activity of a monoclonal antibody targeting the poxvirus A33 protein reveals differences between cell associated and extracellular enveloped virions., PMID:32174513
Protection of mice against the highly pathogenic VVIHD-J by DNA and fowlpox recombinant vaccines, administered by electroporation and intranasal routes, correlates with serum neutralizing activity., PMID:27637905
Systemically administered DNA and fowlpox recombinants expressing four vaccinia virus genes although immunogenic do not protect mice against the highly pathogenic IHD-J vaccinia strain., PMID:24050999
Active vaccination with vaccinia virus A33 protects mice against lethal vaccinia and ectromelia viruses but not against cowpoxvirus; elucidation of the specific adaptive immune response., PMID:23842430
Effect of the deletion of genes encoding proteins of the extracellular virion form of vaccinia virus on vaccine immunogenicity and protective effectiveness in the mouse model., PMID:23785523
CD4+ T cells provide intermolecular help to generate robust antibody responses in vaccinia virus-vaccinated humans., PMID:23667112
L1R, A27L, A33R and B5R vaccinia virus genes expressed by fowlpox recombinants as putative novel orthopoxvirus vaccines., PMID:23578094
Epitope mapping by random peptide phage display reveals essential residues for vaccinia extracellular enveloped virion spread., PMID:23006741
Proteomic basis of the antibody response to monkeypox virus infection examined in cynomolgus macaques and a comparison to human smallpox vaccination., PMID:21209900
The inability of vaccinia virus A33R protein to form intermolecular disulfide-bonded homodimers does not affect the production of infectious extracellular virus., PMID:20947114
Single-shot immunization with recombinant adenovirus encoding vaccinia virus glycoprotein A27L is protective against a virulent respiratory poxvirus infection., PMID:20653083
Protein A33 responsible for antibody-resistant spread of Vaccinia virus is homologous to C-type lectin-like proteins., PMID:20302896
Molecular smallpox vaccine delivered by alphavirus replicons elicits protective immunity in mice and non-human primates., PMID:19833247
Targeting the vaccinia virus L1 protein to the cell surface enhances production of neutralizing antibodies., PMID:18485547
Heterogeneity in the A33 protein impacts the cross-protective efficacy of a candidate smallpox DNA vaccine., PMID:18482742
Differential antigen requirements for protection against systemic and intranasal vaccinia virus challenges in mice., PMID:18448519
Characterization and use of mammalian-expressed vaccinia virus extracellular membrane proteins for quantification of the humoral immune response to smallpox vaccines., PMID:17596428
Characterization of chimpanzee/human monoclonal antibodies to vaccinia virus A33 glycoprotein and its variola virus homolog in vitro and in a vaccinia virus mouse protection model., PMID:17581986
Human T-cell responses to vaccinia virus envelope proteins., PMID:17005679
Subunit recombinant vaccine protects against monkeypox., PMID:16888017
Pathogenicity and immunogenicity in mice of vaccinia viruses mutated in the viral envelope proteins A33R and B5R., PMID:16406098
Immunization with a single extracellular enveloped virus protein produced in bacteria provides partial protection from a lethal orthopoxvirus infection in a natural host., PMID:16256161
Protective immunity to vaccinia virus induced by vaccination with multiple recombinant outer membrane proteins of intracellular and extracellular virions., PMID:15367588
Differential efficacy of vaccinia virus envelope proteins administered by DNA immunisation in protection of BALB/c mice from a lethal intranasal poxvirus challenge., PMID:15308360
Smallpox DNA vaccine protects nonhuman primates against lethal monkeypox., PMID:15078924
Four-gene-combination DNA vaccine protects mice against a lethal vaccinia virus challenge and elicits appropriate antibody responses in nonhuman primates., PMID:12620810
Antibody-sensitive and antibody-resistant cell-to-cell spread by vaccinia virus: role of the A33R protein in antibody-resistant spread., PMID:11752718
Antibody neutralization of the extracellular enveloped form of vaccinia virus., PMID:11162827
The vaccinia virus A33R protein provides a chaperone function for viral membrane localization and tyrosine phosphorylation of the A36R protein., PMID:11119600
DNA vaccination with vaccinia virus L1R and A33R genes protects mice against a lethal poxvirus challenge., PMID:10639319
Neutralizing and protective antibodies directed against vaccinia virus envelope antigens., PMID:9927575
The envelope protein encoded by the A33R gene is required for formation of actin-containing microvilli and efficient cell-to-cell spread of vaccinia virus., PMID:9557708
Extracellular vaccinia virus envelope glycoprotein encoded by the A33R gene., PMID:8648710
For research use only. Not for human or drug use.
