Catalog No.
RXX11701
Expression system
Mammalian Cells
Species reactivity
Shigella flexneri variant Y
Host species
Mouse
Isotype
IgG2a, kappa
Clonality
Monoclonal
Target
S. flexneri Y polysaccharide, [(→2)-α-L-Rha-(1→2)-α-L-Rha-(1→3)-α-L-Rha-(1→3)-β-D-GlcNAc-(1→)]n
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
ChEBI: 59299
Applications
ELISA
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
SYA/J6
Evaluation of a Quadrivalent Shigella flexneri Serotype 2a, 3a, 6, and Shigella sonnei O-Specific Polysaccharide and IpaB MAPS Vaccine., PMID:39460258
High-Throughput Luminescence-Based Serum Bactericidal Assay Optimization and Characterization to Assess Human Sera Functionality Against Multiple Shigella flexneri Serotypes., PMID:39456904
A Novel Shigella O-Polysaccharide-IpaB Conjugate Vaccine Elicits Robust Antibody Responses and Confers Protection against Multiple Shigella Serotypes., PMID:37017547
Effects of Glucosylation and O-Acetylation on the Conformation of Shigella flexneri Serogroup 2 O-Antigen Vaccine Targets., PMID:32204588
Dissemination and serotype modification potential of pSFxv_2, an O-antigen PEtN modification plasmid in Shigella flexneri., PMID:24379081
O-antigen structure of Shigella flexneri serotype Yv and effect of the lpt-O gene variation on phosphoethanolamine modification of S. flexneri O-antigens., PMID:23283000
Molecular dynamics simulations of carbohydrate-mimetic haptens in complex with a complementary anti-carbohydrate antibody., PMID:22789717
Investigation of the binding of a carbohydrate-mimetic peptide to its complementary anticarbohydrate antibody by STD-NMR spectroscopy and molecular-dynamics simulations., PMID:21953925
WaterLOGSY NMR experiments in conjunction with molecular-dynamics simulations identify immobilized water molecules that bridge peptide mimic MDWNMHAA to anticarbohydrate antibody SYA/J6., PMID:21887835
Design, synthesis, and immunochemical characterization of a chimeric glycopeptide corresponding to the Shigella flexneri Y O-polysaccharide and its peptide mimic MDWNMHAA., PMID:19467535
Immunological evidence for functional rather than structural mimicry by a Shigella flexneri Y polysaccharide-mimetic peptide., PMID:18463226
The design, synthesis and evaluation of high affinity macrocyclic carbohydrate inhibitors., PMID:16032350
Synthesis and immunochemical characterization of protein conjugates of carbohydrate and carbohydrate-mimetic peptides as experimental vaccines., PMID:15186860
Saturation-transfer difference NMR studies for the epitope mapping of a carbohydrate-mimetic peptide recognized by an anti-carbohydrate antibody., PMID:14697795
Structural basis of peptide-carbohydrate mimicry in an antibody-combining site., PMID:14645714
Lipopolysaccharide O antigen chains mask IcsA (VirG) in Shigella flexneri., PMID:12725923
Molecular recognition of oligosaccharide epitopes by a monoclonal Fab specific for Shigella flexneri Y lipopolysaccharide: X-ray structures and thermodynamics., PMID:12427018
Strategy for cross-protection among Shigella flexneri serotypes., PMID:9916090
Construction and characterization of genetically-marked bivalent anti-Shigella dysenteriae 1 and anti-Shigella flexneri Y live vaccine candidates., PMID:9188091
Lipopolysaccharide-specific antibodies in plasma and stools of children with Shigella-associated leukemoid reaction and hemolytic-uremic syndrome., PMID:8914761
Towards a vaccine candidate against Shigella dysenteriae 1: expression of the Shiga toxin B-subunit in an attenuated Shigella flexneri aroD carrier strain., PMID:8905616
Specificity of monoclonal antibodies elicited by mucosal infection of BALB/c mice with virulent Shigella flexneri 2a., PMID:8877140
Cross-reactivity of Shigella flexneri serotype 2a O antigen antibodies following immunization or infection., PMID:8879103
Immunoglobulin subclass distribution and dynamics of Shigella-specific antibody responses in serum and stool samples in shigellosis., PMID:7729920
Immune responses in Vietnamese children after a single dose of the auxotrophic, live Shigella flexneri Y vaccine strain SFL124., PMID:8163828
Serum antibody titres to Shigella lipopolysaccharides and invasion plasmid antigens in healthy Costa Rican and Swedish women., PMID:7939434
Synthesis, characterization, and clinical evaluation of conjugate vaccines composed of the O-specific polysaccharides of Shigella dysenteriae type 1, Shigella flexneri type 2a, and Shigella sonnei (Plesiomonas shigelloides) bound to bacterial toxoids., PMID:8359890
Preliminary crystallographic analysis of a Fab specific for the O-antigen of Shigella flexneri cell surface lipopolysaccharide with and without bound saccharides., PMID:8496958
AroD deletion attenuates Shigella flexneri strain 2457T and makes it a safe and efficacious oral vaccine in monkeys., PMID:8356844
Titres of class-specific antibodies against Shigella and Salmonella lipopolysaccharide antigens in colostrum and breast milk of Costa Rican, Swedish and Vietnamese mothers., PMID:1522329
Auxotrophic live oral Shigella flexneri vaccine protects monkeys against challenge with S. flexneri of different serotypes., PMID:1557932
Preparation, characterization, and immunogenicity of conjugates composed of the O-specific polysaccharide of Shigella dysenteriae type 1 (Shiga's bacillus) bound to tetanus toxoid., PMID:1937803
The pathology of Shigella flexneri infection in rhesus monkeys: an endoscopic and histopathological study of colonic lesions., PMID:2191692
Protection of adult rabbits and monkeys from lethal shigellosis by oral immunization with a thymine-requiring and temperature-sensitive mutant of Shigella flexneri Y., PMID:2186582
Use of monoclonal antibodies to type Shigella flexneri in Bangladesh., PMID:2666435
Characterization of five Shigella flexneri variant Y-specific monoclonal antibodies using defined saccharides and glycoconjugate antigens., PMID:2438343
Monoclonal antibodies specific for Shigella flexneri lipopolysaccharides: clones binding to type IV, V, and VI antigens, group 3,4 antigen, and an epitope common to all Shigella flexneri and Shigella dysenteriae type 1 stains., PMID:2437036
Characterization of Shigella flexneri-specific murine monoclonal antibodies by chemically defined glycoconjugates., PMID:3531329
Artificial carbohydrate antigens: a block synthesis of a linear, tetrasaccharide repeating-unit of the Shigella flexneri variant Y polysaccharide., PMID:6200226
For research use only. Not for human or drug use.
