Catalog No.
PHD64201
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human DNASE1 (Gly19-Ala259).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
DNL1,Deoxyribonuclease I,DNASE1,Dornase alfa,Deoxyribonuclease-1,DNase I,DRNI
Purification
Purified by antigen affinity column.
Accession
P24855
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
A dual-acting DNASE1/DNASE1L3 biologic prevents autoimmunity and death in genetic and induced lupus models., PMID:38888971
[Decreased DNase1L3 secretion and associated antibodies induce impaired degradation of NETs in patients with sporadic SLE]., PMID:38246176
Deficiency of macrophage-derived Dnase1L3 causes lupus-like phenotypes in mice., PMID:37804110
Deficiency of macrophage-derived Dnase1L3 causes lupus-like phenotypes in mice., PMID:37131692
Epistatic effects of Siglec-G and DNase1 or DNase1l3 deficiencies in the development of systemic lupus erythematosus., PMID:36969253
Hypercholesterolemia promotes autoantibody production and a lupus-like pathology via decreased DNase-mediated clearance of DNA., PMID:36098213
Neutrophil Extracellular Traps-DNase Balance and Autoimmunity., PMID:34685647
Improved Survival Associated with Local Tumor Response Following Multisite Radiotherapy and Pembrolizumab: Secondary Analysis of a Phase I Trial., PMID:33028595
Neutrophil Extracellular Traps Profiles in Patients with Incident Systemic Lupus Erythematosus and Lupus Nephritis., PMID:31092713
Dnase1-deficient mice spontaneously develop a systemic lupus erythematosus-like disease., PMID:30758851
Dnase1l3 deletion causes aberrations in length and end-motif frequencies in plasma DNA., PMID:30593563
TREX1 is expressed by microglia in normal human brain and increases in regions affected by ischemia., PMID:30062819
[Viewing sepsis and autoimmune disease in relation with infection and NETs-formation]., PMID:29863035
Decreased serum DNase1-activity in patients with autoimmune liver diseases., PMID:28263100
Homogeneous Immunochemical Assay on the Lateral Flow Strip for Measurement of DNase I Activity., PMID:26417694
Human circulating plasma DNA significantly decreases while lymphocyte DNA damage increases under chronic occupational exposure to low-dose gamma-neutron and tritium β-radiation., PMID:26113293
Acute DNase1 treatment improves left ventricular remodeling after myocardial infarction by disruption of free chromatin., PMID:25702039
Impaired degradation and aberrant phagocytosis of necrotic cell debris in the peripheral blood of patients with primary Sjögren's syndrome., PMID:25228497
Safeguarding Stem Cell-Based Regenerative Therapy against Iatrogenic Cancerogenesis: Transgenic Expression of DNASE1, DNASE1L3, DNASE2, DFFB Controlled By POLA1 Promoter in Proliferating and Directed Differentiation Resisting Human Autologous Pluripotent Induced Stem Cells Leads to their Death., PMID:25045589
Eradication of Human Ovarian Cancer Cells by Transgenic Expression of Recombinant DNASE1, DNASE1L3, DNASE2, and DFFB Controlled by EGFR Promoter: Novel Strategy for Targeted Therapy of Cancer., PMID:24587967
Extracellular traps in lipid-rich lesions of carotid atherosclerotic plaques: implications for lipoprotein retention and lesion progression., PMID:24581730
Platelets induce neutrophil extracellular traps in transfusion-related acute lung injury., PMID:22684106
Renal Dnase1 enzyme activity and protein expression is selectively shut down in murine and human membranoproliferative lupus nephritis., PMID:20856893
Risk factors for Aspergillus colonization and allergic bronchopulmonary aspergillosis in children with cystic fibrosis., PMID:20597074
Nuclease deficiencies promote end-stage lupus nephritis but not nephritogenic autoimmunity in (NZB × NZW) F1 mice., PMID:20548325
Deposition of chromatin-IgG complexes in skin of nephritic MRL-lpr/lpr mice is associated with increased local matrix metalloprotease activities., PMID:20500770
Impairment of neutrophil extracellular trap degradation is associated with lupus nephritis., PMID:20439745
Anti-dsDNA antibodies promote initiation, and acquired loss of renal Dnase1 promotes progression of lupus nephritis in autoimmune (NZBxNZW)F1 mice., PMID:20041189
DNase1 activity in systemic lupus erythematosus patients with and without nephropathy., PMID:19844716
Progression of murine lupus nephritis is linked to acquired renal Dnase1 deficiency and not to up-regulated apoptosis., PMID:19528352
Nephritogenic potential of anti-DNA antibodies against necrotic nucleosomes., PMID:19329762
DNase 1 activity in patients with systemic lupus erythematosus: relationship with epidemiological, clinical, immunological and therapeutical features., PMID:19318394
Association of a non-synonymous single-nucleotide polymorphism of DNASEI with SLE susceptibility., PMID:16449364
Chromatin breakdown during necrosis by serum Dnase1 and the plasminogen system., PMID:15188364
Antibody response to Pseudomonas aeruginosa in cystic fibrosis patients: a marker of therapeutic success?--A 30-year cohort study of survival in Danish CF patients after onset of chronic P. aeruginosa lung infection., PMID:15095326
Dehydroepiandrosterone sulfotransferase is a target for transcriptional induction by the vitamin D receptor., PMID:14978251
Pathogenic profiles and molecular signatures of antinuclear autoantibodies rescued from NZM2410 lupus mice., PMID:14757744
Dnase1l3 deficiency in lupus-prone MRL and NZB/W F1 mice., PMID:12974753
Histopathology of lupus-like nephritis in Dnase1-deficient mice in comparison to NZB/W F1 mice., PMID:12220106
Mutation of DNASE1 in people with systemic lupus erythematosus., PMID:11479590
Features of systemic lupus erythematosus in Dnase1-deficient mice., PMID:10835632
Rotavirus VP7 epitope mapping using fragments of VP7 displayed on phages., PMID:10717346
Improved potency of hyperactive and actin-resistant human DNase I variants for treatment of cystic fibrosis and systemic lupus erythematosus., PMID:9660804
Determining the water sorption monolayer of lyophilized pharmaceutical proteins., PMID:9423152
Aerosolized dornase alfa (rhDNase) for therapy of cystic fibrosis., PMID:7881698
Pharmaco-toxicological expert report Pulmozyme rhDNase Genentech, Inc., PMID:8031609
Localization of brush border cytoskeletal proteins in gastric oxynticopeptic cells from the bullfrog Rana catesbeiana., PMID:8111837
Clinical trials of recombinant human DNase in cystic fibrosis patients., PMID:8124306
The analysis of 40 kDa nuclear protein, p40, in interphase cells and mitotic cells., PMID:8308057
Purification and characterization of a DNA-binding protein activated by ionizing radiation., PMID:1587818
For research use only. Not for human or drug use.
