Catalog No.
EVV02501
Expression system
Mammalian Cells
Species
African swine fever virus (isolate Pig/Spain/E-75/1975) (ASFV)
Protein length
Met1-Lys193
Predicted molecular weight
51.34 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
P29999
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
Phosphoprotein p30, p32
An immunoassay based on bioluminescent sensors for rapid detection of African swine fever virus antibodies., PMID:39235247
Development of a P30 protein-based indirect ELISA for detecting African swine fever antibodies utilizing the HEK293F expression system., PMID:38936461
Expression of a recombinant ASFV P30 protein and production of monoclonal antibodies., PMID:37026062
Development of an Effective Double Antigen Sandwich ELISA Based on p30 Protein to Detect Antibodies against African Swine Fever Virus., PMID:36298725
Interaction network of African swine fever virus structural protein p30 with host proteins., PMID:36090090
Antigenicity and immunogenicity of recombinant proteins comprising African swine fever virus proteins p30 and p54 fused to a cell-penetrating peptide., PMID:34715492
A colloidal gold test strip assay for the detection of African swine fever virus based on two monoclonal antibodies against P30., PMID:33495899
Antigenic regions of African swine fever virus phosphoprotein P30., PMID:32145150
Development and characterization of monoclonal antibodies against p30 protein of African swine fever virus., PMID:31129172
Efficient inhibition of African swine fever virus replication by CRISPR/Cas9 targeting of the viral p30 gene (CP204L)., PMID:29362418
Detection of African Swine Fever Virus Antibodies in Serum and Oral Fluid Specimens Using a Recombinant Protein 30 (p30) Dual Matrix Indirect ELISA., PMID:27611939
Recombinant Protein p30 for Serological Diagnosis of African Swine Fever by Immunoblotting Assay., PMID:27390151
Improved Strategy for Molecular Characterization of African Swine Fever Viruses from Sardinia, Based on Analysis of p30, CD2V and I73R/I329L Variable Regions., PMID:27174805
African swine fever virus isolate, Georgia, 2007., PMID:19046509
African swine fever virus protein p30 interaction with heterogeneous nuclear ribonucleoprotein K (hnRNP-K) during infection., PMID:18775702
Optimization and validation of recombinant serological tests for African Swine Fever diagnosis based on detection of the p30 protein produced in Trichoplusia ni larvae., PMID:16954235
Neutralizing antibodies to African swine fever virus proteins p30, p54, and p72 are not sufficient for antibody-mediated protection., PMID:14980493
Antigenic and immunogenic properties of a chimera of two immunodominant African swine fever virus proteins., PMID:11699955
Characterization of p30, a highly antigenic membrane and secreted protein of African swine fever virus., PMID:1604821