Catalog No.
PHK32101
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human RPP25 (Met1-Ala199).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
Ribonuclease P protein subunit p25, RPP25, RNase P protein subunit p25
Purification
Purified by antigen affinity column.
Accession
Q9BUL9
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
PM-Scl and Th/To in systemic sclerosis: a comparison of different autoantibody assays., PMID:33459953
Cancer in Systemic Sclerosis: Analysis of Antibodies Against Components of the Th/To Complex., PMID:33241644
Autoantibodies to a novel Rpp38 (Th/To) derived B-cell epitope are specific for systemic sclerosis and associate with a distinct clinical phenotype., PMID:31323671
Autoantibodies to the mitochondrial RNA processing (MRP) complex also known as Th/To autoantigen., PMID:25462581
Autoantibodies to the Rpp25 component of the Th/To complex are the most common antibodies in patients with systemic sclerosis without antibodies detectable by widely available commercial tests., PMID:24931955
What autoantibody tests should become widely available to help scleroderma diagnosis and management?, PMID:23856077
Rpp25 is a major target of autoantibodies to the Th/To complex as measured by a novel chemiluminescent assay., PMID:23587095
Identity of the RNase MRP- and RNase P-associated Th/To autoantigen., PMID:12483731
Purification and characterization of Rpp25, an RNA-binding protein subunit of human ribonuclease P., PMID:12003489
Rpp14 and Rpp29, two protein subunits of human ribonuclease P., PMID:10024167