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Anti-Human RPP25 Polyclonal Antibody

Catalog #:   PHK32101 Specific References (10) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: Q9BUL9
Overview

Catalog No.

PHK32101

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human RPP25 (Met1-Ala199).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Ribonuclease P protein subunit p25, RPP25, RNase P protein subunit p25

Purification

Purified by antigen affinity column.

Accession

Q9BUL9

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with RPP25 antibody (PHK32101) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 22 kDa
    Observed MW: 22 kDa
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with RPP25 antibody (PHK32101) at 1 μg/ml.

    Lane 1: Marker
    Lane 2: SK-MEL-30

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 21 kDa
    Observed MW: 21 kDa
  • Immunohistochemical
    Immunohistochemical analysis of mouse brain stained for RPP25 with PHK32101.
References

PM-Scl and Th/To in systemic sclerosis: a comparison of different autoantibody assays., PMID:33459953

Cancer in Systemic Sclerosis: Analysis of Antibodies Against Components of the Th/To Complex., PMID:33241644

Autoantibodies to a novel Rpp38 (Th/To) derived B-cell epitope are specific for systemic sclerosis and associate with a distinct clinical phenotype., PMID:31323671

Autoantibodies to the mitochondrial RNA processing (MRP) complex also known as Th/To autoantigen., PMID:25462581

Autoantibodies to the Rpp25 component of the Th/To complex are the most common antibodies in patients with systemic sclerosis without antibodies detectable by widely available commercial tests., PMID:24931955

What autoantibody tests should become widely available to help scleroderma diagnosis and management?, PMID:23856077

Rpp25 is a major target of autoantibodies to the Th/To complex as measured by a novel chemiluminescent assay., PMID:23587095

Identity of the RNase MRP- and RNase P-associated Th/To autoantigen., PMID:12483731

Purification and characterization of Rpp25, an RNA-binding protein subunit of human ribonuclease P., PMID:12003489

Rpp14 and Rpp29, two protein subunits of human ribonuclease P., PMID:10024167

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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Distributor list

For research use only. Not for human or drug use.

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Anti-Human RPP25 Polyclonal Antibody [PHK32101]
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