Catalog No.
PHA28501
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human PRMT5 (Asn295-Gly466).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
PRMT5, SKB1 homolog, 72 kDa ICln-binding protein, Jak-binding protein 1, Histone-arginine N-methyltransferase PRMT5, SKB1Hs, JBP1, Shk1 kinase-binding protein 1 homolog, SKB1, HRMT1L5, IBP72, Protein arginine N-methyltransferase 5
Purification
Purified by antigen affinity column.
Accession
O14744
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Molecular Mechanisms of Tumor Progression and Novel Therapeutic and Diagnostic Strategies in Mesothelioma., PMID:40362535
Discovery of an ApoE4-targeted small-molecule SirT1 enhancer for the treatment of Alzheimer's disease., PMID:40269061
PRMT5 inhibition reduces hyperinflammation in a murine model of secondary hemophagocytic lymphohistiocytosis., PMID:39825858
An immunohistochemical and molecular genetic study of 60 colorectal carcinoma brain metastases in pursuit of predictive biomarkers for cancer therapy., PMID:39824298
PRMT5-Mediated ALKBH5 Methylation Promotes Colorectal Cancer Immune Evasion via Increasing CD276 Expression., PMID:39781264
Time for arginine methylation: PRMT5 inhibition to advance cholangiocarcinoma treatment., PMID:39642967
Assessing the Role of AtGRP7 Arginine 141, a Target of Dimethylation by PRMT5, in Flowering Time Control and Stress Response., PMID:39409642
Identification and validation of anti-protein arginine methyltransferase 5 (PRMT5) antibody as a novel biomarker for systemic sclerosis (SSc)., PMID:38684324
PRMT5 participates in B cell overactivation in patients with primary Sjogren's syndrome (pSS) through RSAD2-mediated NF-κB signaling., PMID:38156384
PRMT blockade induces defective DNA replication stress response and synergizes with PARP inhibition., PMID:38118413
Inhibition of PLK4 remodels histone methylation and activates the immune response via the cGAS-STING pathway in TP53-mutated AML., PMID:37738460
FACS-based genome-wide CRISPR screens define key regulators of DNA damage signaling pathways., PMID:37541219
Phosphorylation of pICln by the autophagy activating kinase ULK1 regulates snRNP biogenesis and splice activity of the cell., PMID:36968021
Expression, Localization and Prognosis Association of MEP50 in Breast Cancer., PMID:36230689
MYBL1 induces transcriptional activation of ANGPT2 to promote tumor angiogenesis and confer sorafenib resistance in human hepatocellular carcinoma., PMID:35987690
Proximity Ligation Assay Allows the Detection, Localization, and Quantification of Protein Arginine Methylation in Fixed Tissue., PMID:35938827
PRMT5 Interacting Partners and Substrates in Oligodendrocyte Lineage Cells., PMID:35370564
Dermatan Sulfate Is a Potential Regulator of IgH via Interactions With Pre-BCR, GTF2I, and BiP ER Complex in Pre-B Lymphoblasts., PMID:34113352
Pan-methylarginine antibody generation using PEG linked GAR motifs as antigens., PMID:34107353
Symmetrically dimethylated histone H3R2 promotes global transcription during minor zygotic genome activation in mouse pronuclei., PMID:33980975
miR103a-3p in extracellular vesicles from FcεRI-aggregated human mast cells enhances IL-5 production by group 2 innate lymphoid cells., PMID:33465368
The Oncometabolite 5'-Deoxy-5'-Methylthioadenosine Blocks Multiple Signaling Pathways of NK Cell Activation., PMID:33123121
Actin R256 Mono-methylation Is a Conserved Post-translational Modification Involved in Transcription., PMID:32997990
The Protein Arginine Methyltransferases 1 and 5 affect Myc properties in glioblastoma stem cells., PMID:31685892
Using proximity ligation assay to detect protein arginine methylation., PMID:31499160
PRMT5 is upregulated by B-cell receptor signaling and forms a positive-feedback loop with PI3K/AKT in lymphoma cells., PMID:31123343
Maintenance of SOX9 stability and ECM homeostasis by selenium-sensitive PRMT5 in cartilage., PMID:30858101
PRMT5 Associates With the FOXP3 Homomer and When Disabled Enhances Targeted p185erbB2/neu Tumor Immunotherapy., PMID:30800128
Coprs inactivation leads to a derepression of LINE1 transposons in spermatocytes., PMID:30652083
PRMT5 is essential for B cell development and germinal center dynamics., PMID:30604754
Overexpression of HOXC10 promotes angiogenesis in human glioma via interaction with PRMT5 and upregulation of VEGFA expression., PMID:30429891
A simplified characterization of S-adenosyl-l-methionine-consuming enzymes with 1-Step EZ-MTase: a universal and straightforward coupled-assay for in vitro and in vivo setting., PMID:29449933
Identifying the ubiquitination targets of E6AP by orthogonal ubiquitin transfer., PMID:29263404
Novel insights into mesothelioma biology and implications for therapy., PMID:28740119
A Genetic Variant Ameliorates β-Thalassemia Severity by Epigenetic-Mediated Elevation of Human Fetal Hemoglobin Expression., PMID:28669403
The protein arginine methyltransferase 5 promotes malignant phenotype of hepatocellular carcinoma cells and is associated with adverse patient outcomes after curative hepatectomy., PMID:28101581
Subsets of Visceral Adipose Tissue Nuclei with Distinct Levels of 5-Hydroxymethylcytosine., PMID:27171244
The proteomic investigation reveals interaction of mdig protein with the machinery of DNA double-strand break repair., PMID:26293673
Prmt5 is a regulator of muscle stem cell expansion in adult mice., PMID:26028225
PRMT9 is a type II methyltransferase that methylates the splicing factor SAP145., PMID:25737013
The Sm protein methyltransferase PRMT5 is not required for primordial germ cell specification in mice., PMID:25519955
Human fetal globin gene expression is regulated by LYAR., PMID:25092918
Expression of proteins involved in epigenetic regulation in human cutaneous melanoma and peritumoral skin., PMID:24850177
PDT-induced epigenetic changes in the mouse cerebral cortex: a protein microarray study., PMID:24055374
Role of type II protein arginine methyltransferase 5 in the regulation of Circadian Per1 gene., PMID:23133559
Regulation of post-translational protein arginine methylation during HeLa cell cycle., PMID:20541591
Native purification of protein and RNA-protein complexes using a novel affinity procedure., PMID:19690462
Proteomic analysis of murine Piwi proteins reveals a role for arginine methylation in specifying interaction with Tudor family members., PMID:19584108
GANP suppresses the arginine methyltransferase PRMT5 regulating IL-4-mediated STAT6-signaling to IgE production in B cells., PMID:19181385
Mutations in the Type II protein arginine methyltransferase AtPRMT5 result in pleiotropic developmental defects in Arabidopsis., PMID:17573539
For research use only. Not for human or drug use.
