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Anti-Human CD277/BTN3A1 Polyclonal Antibody

Catalog #:   PHA13501 Specific References (16) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: O00481
Overview

Catalog No.

PHA13501

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CD277/BTN3A1 (Gln30-Ser236).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

BTF5, BTN3A1, Butyrophilin subfamily 3 member A1, CD277

Purification

Purified by antigen affinity column.

Accession

O00481

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with CD277/BTN3A1 antibody (PHA13501) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 24 kDa
    Observed MW: 24 kDa
References

CD277 agonist enhances the immunogenicity of relapsed/refractory acute myeloid leukemia towards Vδ2+ T cell cytotoxicity., PMID:35920929

BTN3A1 governs antitumor responses by coordinating αβ and γδ T cells., PMID:32820120

γ9δ2T cell diversity and the receptor interface with tumor cells., PMID:32484803

Alpaca (Vicugna pacos), the first nonprimate species with a phosphoantigen-reactive Vγ9Vδ2 T cell subset., PMID:32139608

Immunologic Consequences of Sequencing Cancer Radiotherapy and Surgery., PMID:30964698

Regulation of Human γδ T Cells by BTN3A1 Protein Stability and ATP-Binding Cassette Transporters., PMID:29670629

The butyrophilin 3A1 intracellular domain undergoes a conformational change involving the juxtamembrane region., PMID:28705810

BTN3A1-antibodies and phosphoantigens: TCRVγ9Vδ2 "see" the difference., PMID:28597565

Butyrophilin 3A (BTN3A, CD277)-specific antibody 20.1 differentially activates Vγ9Vδ2 TCR clonotypes and interferes with phosphoantigen activation., PMID:28386905

BTN3A molecules considerably improve Vγ9Vδ2T cells-based immunotherapy in acute myeloid leukemia., PMID:27853633

Butyrophilin 3A/CD277-Dependent Activation of Human γδ T Cells: Accessory Cell Capacity of Distinct Leukocyte Populations., PMID:27619996

Phosphoantigens and butyrophilin 3A1 induce similar intracellular activation signaling in human TCRVγ9+ γδ T lymphocytes., PMID:24925024

Butyrophilin 3A1 plays an essential role in prenyl pyrophosphate stimulation of human Vγ2Vδ2 T cells., PMID:23833237

The molecular basis for modulation of human Vγ9Vδ2 T cell responses by CD277/butyrophilin-3 (BTN3A)-specific antibodies., PMID:22846996

Key implication of CD277/butyrophilin-3 (BTN3A) in cellular stress sensing by a major human γδ T-cell subset., PMID:22767497

Differential role for CD277 as a co-regulator of the immune signal in T and NK cells., PMID:21918970

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

Contact Information

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Distributor list

For research use only. Not for human or drug use.

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Anti-Human CD277/BTN3A1 Polyclonal Antibody [PHA13501]
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