Catalog No.
PHD12101
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human CYP11B1 (Gly134-Asn503).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
CYP11B1,Cytochrome P-450c11,Steroid 11-beta-hydroxylase,CYP11B1,Cytochrome P450C11,CYPXIB1,Cytochrome P450 11B1,mitochondrial,S11BH
Purification
Purified by antigen affinity column.
Accession
P15538
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Immunohistochemical Analysis of CYP11B2 (Aldosterone Synthase) and Evaluation of the HISTALDO Classification in Patients with Primary Aldosteronism., PMID:40325812
Diagnostic and prognostic assessments of adrenocortical carcinomas by pathological features, immunohistochemical markers and reticular histochemistry staining., PMID:38802933
Immunohistochemical staining seems mandatory for individualizing and shortening follow-up in unilateral primary aldosteronism., PMID:37525427
Loss of epidermal glucocorticoid receptor protects against whole body metabolic dysfunction upon chronic corticosterone treatment., PMID:37364709
Local biosynthesis of corticosterone in rat skeletal muscle., PMID:32437963
Loss of Cyp11c1 causes delayed spermatogenesis due to the absence of 11-ketotestosterone., PMID:31910154
Synchronous aldosterone- and cortisol-producing adrenocortical adenomas diagnosed using CYP11B immunohistochemistry., PMID:31666955
H-score of 11β-hydroxylase and aldosterone synthase in the histopathological diagnosis of adrenocortical tumors., PMID:31332713
Impact of immunohistochemistry on the diagnosis and management of primary aldosteronism: An important tool for improved patient follow-up., PMID:30654730
Icariin inhibits inflammation via immunomodulation of the cutaneous hypothalamus-pituitary-adrenal axis in vitro., PMID:30155911
MRAP deficiency impairs adrenal progenitor cell differentiation and gland zonation., PMID:29879378
Histopathological and genetic characterization of aldosterone-producing adenomas with concurrent subclinical cortisol hypersecretion: a case series., PMID:28405879
Disordered zonal and cellular CYP11B2 enzyme expression in familial hyperaldosteronism type 3., PMID:27793677
Immunohistochemistry of aldosterone synthase leads the way to the pathogenesis of primary aldosteronism., PMID:27751767
Immunohistochemistry of the adrenal in primary aldosteronism., PMID:27119751
Intratumoral heterogeneity of steroidogenesis in aldosterone-producing adenoma revealed by intensive double- and triple-immunostaining for CYP11B2/B1 and CYP17., PMID:26597777
Adrenal CYP11B1/2 expression in primary aldosteronism: immunohistochemical analysis using novel monoclonal antibodies., PMID:24837548
Development of monoclonal antibodies against human CYP11B1 and CYP11B2., PMID:24325867
Testosterone implants modify the steroid hormone balance and the gonadal physiology of gilthead seabream (Sparus aurata L.) males., PMID:23743364
Histopathological diagnosis of primary aldosteronism using CYP11B2 immunohistochemistry., PMID:23443813
Immunohistochemistry improves histopathologic diagnosis in primary aldosteronism., PMID:23436930
Comparison of serum cortisol measurement by immunoassay and liquid chromatography-tandem mass spectrometry in patients receiving the 11β-hydroxylase inhibitor metyrapone., PMID:21813575
Adrenocortical zonation in humans under normal and pathological conditions., PMID:20200334
ACTH receptor blockade: a novel approach to treat congenital adrenal hyperplasia, or Cushing's disease., PMID:19931309
Physiological and pathophysiological applications of sensitive ELISA methods for urinary deoxycorticosterone and corticosterone in rodents., PMID:19577584
Development of a highly sensitive ELISA for aldosterone in mouse urine: validation in physiological and pathophysiological states of aldosterone excess and depletion., PMID:19162057
Analyzing the functional and structural consequences of two point mutations (P94L and A368D) in the CYP11B1 gene causing congenital adrenal hyperplasia resulting from 11-hydroxylase deficiency., PMID:16670167
Zone-specific cell proliferation during adrenocortical regeneration after enucleation in rats., PMID:15972274
Expression of 11beta-hydroxylase in rat Leydig cells., PMID:11796518
Serum 18-hydroxycortisol in primary aldosteronism, hypertension, and normotensives., PMID:11566957
Expression of 11beta-hydroxylase and aldosterone synthase genes in the rat brain., PMID:10828825
Complex formation in vesicle-reconstituted mitochondrial cytochrome P450 systems (CYP11A1 and CYP11B1) as evidenced by rotational diffusion experiments using EPR and ST-EPR., PMID:10413522
Implication of ZOG protein (zona glomerulosa-specific protein) in zone development of the adrenal cortex., PMID:9888532
A novel stage-specific antigen is expressed only in early stages of spermatogonia in Japanese eel, Anguilla japonica testis., PMID:9820193
Immunochemical characterization of steroid hydroxylases of adrenocortical mitochondria. 1. Antibodies as inhibitors of cytochrome P450scc (CYP11A1) and P450(11 beta) (CYP11B1) activities., PMID:9481871
Some effects of a low sodium intake on the expression of P450 aldosterone synthase in the hamster adrenal cortex: immunoblotting, immunofluorescent and immuno-gold electron microscopic studies., PMID:8708547
The hamster adrenal cytochrome P450C11 has equipotent 11beta-hydroxylase and 19-hydroxylase activities, but no aldosterone synthase activity., PMID:8645611
Steroidogenic enzyme P450c17 is expressed in the embryonic central nervous system., PMID:7588260
P450aldo in hamster adrenal cortex: immunofluorescent and immuno-gold electron microscopic studies., PMID:7588390
Differential short- and long-term effects of insulin on ANG II action in human adrenal glomerulosa cells., PMID:7840166
Identification of a cyclic adenosine 3',5'-monophosphate-response element in the rat aromatase promoter that is required for transcriptional activation in rat granulosa cells and R2C leydig cells., PMID:7854348
The presence of two cytochrome P450 aldosterone synthase mRNAs in the hamster adrenal., PMID:8031709
Immunological analysis of developmental changes in ecdysone 20-mono-oxygenase expression in the cotton leafworm, Spodoptera littoralis., PMID:8192659
Autoantibody epitope mapping of the 21-hydroxylase antigen in autoimmune Addison's disease., PMID:7513715
Electron microscopic immunocytochemistry on the colocalization of ATP synthase and cytochrome P-450 of side chain cleavage enzymes in mitochondria of rat and bovine adrenal cortical cells., PMID:8373664
Steroid 11ß-hydroxylation by a fungal microsomal cytochrome P450., PMID:22217857
Induction and characterization of cytochromes P450IA and -IIB in the newt, Pleurodeles waltl., PMID:1280580
Effect of vasotocin on cytosolic free calcium concentrations in frog adrenocortical cells in primary culture., PMID:1505452
Expression of 17 alpha-hydroxylase and 3 beta-hydroxysteroid dehydrogenase in fetal human adrenocortical cells transfected with SV40 T antigen., PMID:1325152
21-Hydroxylase, a major autoantigen in idiopathic Addison's disease., PMID:1351548
For research use only. Not for human or drug use.
