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Anti-Human AOX1 Polyclonal Antibody

Catalog #:   PHG08401 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, IF, WB
Accession: Q06278
Overview

Catalog No.

PHG08401

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human AOX1 (Met1-Lys166).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, IF:1:50-1:200, WB: 1:1000-1:4000

Target

Azaheterocycle hydroxylase, AO, AOX1, Aldehyde oxidase, Aldehyde oxidase 1

Concentration

1.98 mg/ml

Purification

Purified by antigen affinity column.

Accession

Q06278

Applications

ELISA, IHC, IF, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with AOX1 antibody (PHG08401) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 20 kDa
    Observed MW: 20 kDa
References

The clock component LHY1b negatively regulates alkaline stress by repressing AOX1-mediated oxidative responses in soybean., PMID:40513111

Production of codon-optimized Human papillomavirus type 52 L1 virus-like particles in Pichia pastoris BG10 expression system., PMID:35302435

Correction: Epigenetic loss of AOX1 expression via EZH2 leads to metabolic deregulations and promotes bladder cancer progression., PMID:32820250

An efficient constitutive expression system for Anti-CEACAM5 nanobody production in the yeast Pichia pastoris., PMID:30414968

[Secreted expression of Japanese encephalitis virus prME in Pichia pastoris and immunogenicity evaluation of the virus-like particles in mice]., PMID:28876040

Process development for production and purification of the Schistosoma mansoni Sm14 antigen., PMID:28389350

Expressing anti-HIV VRC01 antibody using the murine IgG1 secretion signal in Pichia pastoris., PMID:28342171

Bioprocess development for extracellular production of recombinant human interleukin-3 (hIL-3) in Pichia pastoris., PMID:27514666

Yeast Surface Display of Two Proteins Previously Shown to Be Protective Against White Spot Syndrome Virus (WSSV) in Shrimp., PMID:26083446

Whole recombinant Pichia pastoris expressing HPV16 L1 antigen is superior in inducing protection against tumor growth as compared to killed transgenic Leishmania., PMID:25668661

Cloning, expression and purification of squalene synthase from Candida tropicalis in Pichia pastoris., PMID:24184232

Extending the serum half-life of G-CSF via fusion with the domain III of human serum albumin., PMID:24151579

Regulation of alcohol oxidase 1 (AOX1) promoter and peroxisome biogenesis in different fermentation processes in Pichia pastoris., PMID:23735484

Identification, expression, and taxonomic distribution of alternative oxidases in non-angiosperm plants., PMID:23664893

High-level expression and immunogenicity of a porcine circovirus type 2 capsid protein through codon optimization in Pichia pastoris., PMID:23143467

Expression, purification and characterization of recombinant human interleukin-2-serum albumin (rhIL-2-HSA) fusion protein in Pichia pastoris., PMID:22609631

Improvement of N-glycan site occupancy of therapeutic glycoproteins produced in Pichia pastoris., PMID:22569635

Cloning and expression of Toxoplasma gondii dense granular protein 4 (GRA4) in Pichia pastoris., PMID:21399595

Single-chain antibody fragment production in Pichia pastoris: Benefits of prolonged pre-induction glycerol feeding., PMID:21259439

Expression and purification of biologically active Trichoderma virens proteinaceous elicitor Sm1 in Pichia pastoris., PMID:20233605

Expression of recombinant proteins in the methylotrophic yeast Pichia pastoris., PMID:20186119

High-level expression of a phage display-derived scFv in Pichia pastoris., PMID:19554300

Over expression and analysis of O-glycosylated recombinant human granulocyte colony stimulating factor in Pichia pastoris using Agilent 2100 Bioanalyzer., PMID:19527756

Cloning of a gene encoding dextranase from Lipomyces starkeyi and its expression in Pichia pastoris., PMID:19307767

Biochemical and structural characterization of RHDV capsid protein variants produced in Pichia pastoris: advantages for immunization strategies and vaccine implementation., PMID:18824033

Expression, purification, and characterization of recombinant human serum albumin fusion protein with two human glucagon-like peptide-1 mutants in Pichia pastoris., PMID:18556214

A33scFv-green fluorescent protein, a recombinant single-chain fusion protein for tumor targeting., PMID:18033820

Expression in Pichia pastoris and immunological evaluation of a truncated Dengue envelope protein., PMID:17401146

High level expression of a promising anti-idiotypic antibody fragment vaccine against HIV-1 in Pichia pastoris., PMID:17270302

Engineering of Pichia pastoris for improved production of antibody fragments., PMID:16570317

Oxygen-limited control of methanol uptake for improved production of a single-chain antibody fragment with recombinant Pichia pastoris., PMID:16532314

Impact of methanol concentration on secreted protein production in oxygen-limited cultures of recombinant Pichia pastoris., PMID:16320364

Reduced oxygen supply increases process stability and product yield with recombinant Pichia pastoris., PMID:15575710

An optimized fermentation process for high-level production of a single-chain Fv antibody fragment in Pichia pastoris., PMID:15294276

Increasing secretion of a bivalent anti-T-cell immunotoxin by Pichia pastoris., PMID:15184133

Recombinant human serum amyloid P component from Pichia pastoris: production and characterization., PMID:15135404

Expression of a novel regenerating gene product, Reg IV, by high density fermentation in Pichia pastoris: production, purification, and characterization., PMID:14550637

High-level production in Pichia pastoris of an anti-p185HER-2 single-chain antibody fragment using an alternative secretion expression vector., PMID:12962542

Expression of TIMP-1 in Pichia pastoris. Selection of an anti-TIMP-1 specific single-chain Fv antibody from a large non-immune library., PMID:12482633

Gene optimization is necessary to express a bivalent anti-human anti-T cell immunotoxin in Pichia pastoris., PMID:12135560

Expression and purification of Dengue virus type 2 envelope protein as a fusion with hepatitis B surface antigen in Pichia pastoris., PMID:11570849

Analysis of single-chain antibody production in Pichia pastoris using on-line methanol control in fed-batch and mixed-feed fermentations., PMID:11410859

Molecular cloning and high-level expression of G2 protein of hantaan (HTN) virus 76-118 strain in the yeast Pichia pastoris KM71., PMID:11324753

Alcohol oxidase is a novel pathogenicity factor for Cladosporium fulvum, but aldehyde dehydrogenase is dispensable., PMID:11277434

A recombinant group 1 house dust mite allergen, rDer f 1, with biological activities similar to those of the native allergen., PMID:11087686

Comparative expression and purification of human glutamic acid decarboxylase from Saccharomyces cerevisiae and Pichia pastoris., PMID:10862868

Production and detailed characterization of biologically active olive pollen allergen Ole e 1 secreted by the yeast Pichia pastoris., PMID:10215867

Protection of mice against a lethal influenza virus challenge after immunization with yeast-derived secreted influenza virus hemagglutinin., PMID:10091596

Role of glycosylation in the functional expression of an Aspergillus niger phytase (phyA) in Pichia pastoris., PMID:10087168

Expression of enzymatically active, recombinant barley alpha-glucosidase in yeast and immunological detection of alpha-glucosidase from seed tissue., PMID:9747846

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-Human AOX1 Polyclonal Antibody [PHG08401]
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