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Anti-Human DNA2 Polyclonal Antibody

Catalog #:   PHE82701 Specific References (32) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P51530
Overview

Catalog No.

PHE82701

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human DNA2 (Met1-Arg599).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

DNA replication ATP-dependent helicase-like homolog, KIAA0083, hDNA2, DNA2, DNA2L, DNA replication ATP-dependent helicase/nuclease DNA2

Purification

Purified by antigen affinity column.

Accession

P51530

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with DNA2 antibody (PHE82701) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 71 kDa
    Observed MW: 71 kDa
References

DNA Conformation-Regulated Hemin Switch for Lab-on-Chip Chemiluminescent Detection of an Antibody Secreted from Hybridoma Cells., PMID:39503400

One-tube B7-H3 detection based on isothermal exponential amplification and dendritic hybridization chain reaction., PMID:38633035

Electrochemical biosensing for E.coli detection based on triple helix DNA inhibition of CRISPR/Cas12a cleavage activity., PMID:38057050

Proximity hybridization regulated dual-mode ratiometric biosensor for estriol detection in pregnancy serum., PMID:37709442

Washing-free electrogenerated chemiluminescence magnetic microbiosensors based on target assistant proximity hybridization for multiple protein biomarkers., PMID:36965986

Universal point-of-care detection of proteins based on proximity hybridization-mediated isothermal exponential amplification., PMID:35332897

Multiple DSB Resection Activities Redundantly Promote Alternative End Joining-Mediated Class Switch Recombination., PMID:34926456

A general scattering proximity immunoassay with the formation of dimer of gold nanoparticle., PMID:34215130

A novel electrochemical immunosensor for hepatitis B surface antigen based on Fe3O4 nanoflowers and heterogeneous chain reaction signal amplification strategy., PMID:33076081

Specificity of end resection pathways for double-strand break regions containing ribonucleotides and base lesions., PMID:32555206

Electrochemical detection of β-lactoglobulin based on a highly selective DNA aptamer and flower-like Au@BiVO4 microspheres., PMID:32475386

Ubiquitinated-PCNA protects replication forks from DNA2-mediated degradation by regulating Okazaki fragment maturation and chromatin assembly., PMID:32358495

An anchored monopodial DNA walker triggered by proximity hybridization for amplified amperometric biosensing of nucleic acid and protein., PMID:32200901

Separation-Free Electrogenerated Chemiluminescence Immunoassay Incorporating Target Assistant Proximity Hybridization and Dynamically Competitive Hybridization of a DNA Signal Probe., PMID:31808341

Dna2 nuclease deficiency results in large and complex DNA insertions at chromosomal breaks., PMID:30518856

An ultrasensitive electrochemical immunoassay based on a proximity hybridization-triggered three-layer cascade signal amplification strategy., PMID:30465676

Highly sensitive electrochemical immunosensor for golgi protein 73 based on proximity ligation assay and enzyme-powered recycling amplification., PMID:30327105

Electrochemiluminescent Imaging for Multi-immunoassay Sensitized by Dual DNA Amplification of Polymer Dot Signal., PMID:29847924

Proximity Hybridization-Regulated Immunoassay for Cell Surface Protein and Protein-Overexpressing Cancer Cells via Electrochemiluminescence., PMID:29433314

Target-induced cyclic DNAzyme formation for colorimetric and chemiluminescence imaging assay of protein biomarkers., PMID:28879360

Highly Effective Protein Converting Strategy for Ultrasensitive Electrochemical Assay of Cystatin C., PMID:27104623

Homogeneous electrochemical immunoassay of aflatoxin B1 in foodstuff using proximity-hybridization-induced omega-like DNA junctions and exonuclease III-triggered isothermal cycling signal amplification., PMID:26960904

Target-regulated proximity hybridization with three-way DNA junction for in situ enhanced electronic detection of marine biotoxin based on isothermal cycling signal amplification strategy., PMID:25747510

Immunoreaction-triggered DNA assembly for one-step sensitive ratiometric electrochemical biosensing of protein biomarker., PMID:25460904

A novel signal-on strategy for M.SssI methyltransfease activity analysis and inhibitor screening based on photoelectrochemical immunosensor., PMID:25460890

Strip biosensor for amplified detection of nerve growth factor-beta based on a molecular translator and catalytic DNA circuit., PMID:25068151

Ratiometric electrochemical proximity assay for sensitive one-step protein detection., PMID:24618513

Time-resolved fluorescence based DNA detection using novel europium ternary complex doped silica nanoparticles., PMID:19836584

Design parameters to control synthetic gene expression in Escherichia coli., PMID:19759823

Human Dna2 is a nuclear and mitochondrial DNA maintenance protein., PMID:19487465

Dynamic localization of an Okazaki fragment processing protein suggests a novel role in telomere replication., PMID:12024033

Novel DNA-protein complex and a large DNA in SLE cryoprecipitates., PMID:3802574

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-Human DNA2 Polyclonal Antibody [PHE82701]
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