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Anti-Human CUL2 Polyclonal Antibody

Catalog #:   PHG61301 Specific References (11) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: Q13617
Overview

Catalog No.

PHG61301

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CUL2 (Thr526-Ala745).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Cullin-2, CUL2, CUL-2

Purification

Purified by antigen affinity column.

Accession

Q13617

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with CUL2 antibody (PHG61301) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 28 kDa
    Observed MW: 28 kDa
References

Activity-based profiling of cullin-RING E3 networks by conformation-specific probes., PMID:37653169

Activity-based profiling of cullin-RING ligase networks by conformation-specific probes., PMID:36711970

Butyrated ManNAc analog improves protein expression in Chinese hamster ovary cells., PMID:29427449

Regulation of the MEI-1/MEI-2 Microtubule-Severing Katanin Complex in Early Caenorhabditis elegans Development., PMID:27527792

Cullin-5 and cullin-2 play a role in the development of neuromuscular junction and the female germ line of Drosophila., PMID:21869472

Adenovirus 12 E4orf6 inhibits ATR activation by promoting TOPBP1 degradation., PMID:20566845

NEDD8 acts as a 'molecular switch' defining the functional selectivity of VHL., PMID:18323857

The COP9 signalosome inhibits p27(kip1) degradation and impedes G1-S phase progression via deneddylation of SCF Cul1., PMID:11967155

In vitro ubiquitination of cyclin D1 by ROC1-CUL1 and ROC1-CUL3., PMID:11311237

CUL-2 is required for the G1-to-S-phase transition and mitotic chromosome condensation in Caenorhabditis elegans., PMID:10587644

The von Hippel-Lindau tumor suppressor gene product promotes, but is not essential for, NEDD8 conjugation to cullin-2., PMID:10585493

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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Distributor list

For research use only. Not for human or drug use.

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Anti-Human CUL2 Polyclonal Antibody [PHG61301]
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