Catalog No.
PHB22001
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human PSIP1 (Thr2-Asn87).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
Transcriptional coactivator p75/p52, CLL-associated antigen KW-7, PSIP1, DFS70, Dense fine speckles 70 kDa protein, DFS 70, PC4 and SFRS1-interacting protein, LEDGF, PSIP2, Lens epithelium-derived growth factor
Purification
Purified by antigen affinity column.
Accession
O75475
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Anti-C1s autoantibodies as complementary serologic biomarker in lupus nephritis., PMID:40132784
Anti-aquaporin-5 and anti-poly-U-binding-factor-60kDa protein antibodies in primary Sjögren's disease patients: preliminary data and correlation with disease activity indices., PMID:39699867
Macaque liver substrate for evaluating dense fine speckled-like patterns on HEp2010 cells., PMID:39168779
Clinical Significance of Antibodies to DFS70 in Immunoinflammatory Rheumatic Diseases., PMID:38861147
Clinical features of patients with connective tissue disease with anti-human upstream binding factor antibodies: A single-center retrospective study., PMID:38421809
Anti-DFS70 Antibodies Are Associated With Proliferative Lupus Nephritis and Renal Pathological Activity., PMID:35185903
The LEDGF/p75 Integrase Binding Domain Interactome Contributes to the Survival, Clonogenicity, and Tumorsphere Formation of Docetaxel-Resistant Prostate Cancer Cells., PMID:34685704
Prevalence of anti-dense fine speckled 70 antibodies in healthy individuals and patients with antinuclear antibody-associated autoimmune rheumatic diseases in Japan., PMID:33655922
Stratification of Nuclear Homogeneous Patterns on HEp-2 Cells Based on Neutrophil Nuclear Staining., PMID:33537219
Anti-DFS70 Antibodies Among Patient and Healthy Population Cohorts in China: Results From a Multicenter Training Program Showing Spontaneous Abortion and Pediatric Systemic Autoimmune Rheumatic Diseases Are Common in Anti-DFS70 Positive Patients., PMID:33133072
The antinuclear antibody dense fine speckled pattern and possible clinical associations: An indication of a proinflammatory microenvironment., PMID:33121975
Monospecific anti-DFS70 antibodies are moderately helpful in excluding ANA-associated rheumatic disease in patients presenting with a dense fine speckled pattern - A systematic review and meta-analysis of diagnostic test accuracy., PMID:32801047
High Sensitivity Detection of Anti-DFS70 Antibodies by Radioimmunoprecipitation Assay (RIPA)., PMID:32390377
Anti-DFS70 antibodies detected by specific methods in patients with thrombosis or recurrent pregnancy loss: no evidence of an association., PMID:32385308
Assessing vitamin D levels in an anti-DFS70 positive population: New insights emerging., PMID:32164458
Twenty years of research on the DFS70/LEDGF autoantibody-autoantigen system: many lessons learned but still many questions., PMID:32127038
Prevalence of anti-DFS70 autoantibodies in a Latin American cohort of patients with systemic lupus erythematosus and without autoimmune diseases., PMID:32086623
Clinical value of anti-DFS70 antibodies in a cohort of patients undergoing routine antinuclear antibodies testing., PMID:32057435
A panel of autoantibodies against tumor-associated antigens in the early immunodiagnosis of lung cancer., PMID:31980218
Discovering novel lung cancer associated antigens and the utilization of their autoantibodies in detection of lung cancer., PMID:31839396
Are Anti-DFS70 Autoantibodies Protective?, PMID:31474007
Clinical Aspects of the Dense Fine Speckled Pattern in Indirect Immunofluorescence-Antinuclear Antibody Screening and Its Association with DFS70 Autoantibodies., PMID:31471339
Investigation of anti-DFS70 antibody in patients with systemic autoimmune rheumatic diseases., PMID:31396837
Negative specificity for DSF70 in ANA positive leprosy sera., PMID:31278511
Anti-DFS70 among HIV-positive individuals - A prospective study., PMID:31174828
Clinical use of anti-DFS70 autoantibodies., PMID:30972541
Utility of Anti-DSF70 Antibodies to Predict Connective Tissue Disease in Patients Originally Presenting with Idiopathic Interstitial Pneumonia., PMID:30928980
The long-awaited 'pseudo-DFS pattern'., PMID:30879347
Polymorphism rs2395655 affects LEDGF/p75 binding activity and p21WAF1/CIP1 gene expression in esophageal squamous cell carcinoma., PMID:30854807
Prevalence and serological profile of anti-DFS70 positive subjects from a routine ANA cohort., PMID:30778125
Increased prevalence of anti-DFS70 antibodies in young females: experience from a large international multi-center study on blood donors., PMID:30739097
Anti-DFS70 antibodies: an update on our current understanding and their clinical usefulness., PMID:30623687
Improved accuracy in DFS pattern interpretation using a novel HEp-2 ELITE system., PMID:30617598
Revisiting the antinuclear antibody test with emphasis on a new pattern: anti-DFS70 antibody., PMID:30609056
[Investigation of the diagnostic value of anti-dense fine speckled 70/lens epithelium derived growth factor p75 autoantibody for autoimmune diseases]., PMID:30522426
A better definition of the anti-DFS70 antibody screening by IIF methods., PMID:30017652
Evaluation of Samples with DFS Staining Pattern Detected by Indirect Immunofluorescence Assay., PMID:29739116
New genetically engineered DFS70 knock-out HEp-2 cells enable rapid and specific recognition of anti-DFS70 antibodies., PMID:29732925
The importance of detecting anti-DFS70 in routine clinical practice: comparison of different care settings., PMID:29427547
Prevalence and Clinical Significance of Anti-DFS70 in Antinuclear Antibody (ANA)-positive Patients Undergoing Routine ANA Testing in a New Zealand Public Hospital., PMID:29419453
Simultaneous Distinction of Monospecific and Mixed DFS70 Patterns During ANA Screening with a Novel HEp-2 ELITE/DFS70 Knockout Substrate., PMID:29364249
Clinical significance of anti-dense fine speckled 70 antibody in patients with fibromyalgia., PMID:29166758
Prevalence of anti-DFS70 antibodies in patients with and without systemic autoimmune rheumatic diseases., PMID:28770702
The prevalence and determinants of anti-DFS70 autoantibodies in an international inception cohort of systemic lupus erythematosus patients., PMID:28420054
The clinical impact of Anti-DFS70 antibodies in undifferentiated connective tissue disease: case reports and a review of the literature., PMID:27427300
Anti-DFS70 antibodies detected by immunoblot methods: A reliable tool to confirm the dense fine speckles ANA pattern., PMID:27374867
The Clinical Relevance of Anti-DFS70 Autoantibodies., PMID:27350273
Development and multi-center evaluation of a novel immunoadsorption method for anti-DFS70 antibodies., PMID:27252267
Autoantibodies Against DFS70/LEDGF Exclusion Markers for Systemic Autoimmune Rheumatic Diseases (SARD)., PMID:27215068
[Antinuclear antibodies without connective tissue disease : Antibodies against LEDGF/DSF70]., PMID:26820723
For research use only. Not for human or drug use.
