Please ensure Javascript is enabled for purposes of website accessibility
Home / Products / Antibody / Polyclonal Antibody

Anti-CCNB1 Polyclonal Antibody (PHD05701)

Host species:Rabbit
Isotype:IgG
Applications:ELISA, IHC, WB
Accession:P14635
Overview

Catalog No.

PHD05701

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CCNB1 (Met1-Pro91).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

CCNB1,G2/mitotic-specific cyclin-B1,CCNB

Purification

Purified by antigen affinity column.

Accession

P14635

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with CCNB1 antibody (PHD05701) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 12 kDa
    Observed MW: 12 kDa
  • Immunocytochemistry/ Immunofluorescence
    CCNB1 in Hela Cell Line.
    The Hela cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with CCNB1 (PHD05701) at 18.6 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-594) preabsorbed at 1/100 dilution (Shown in red) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).
References
Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

Contact Information

Order: order@antibodysystem.com

Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

Need help with your order?

Find out more about placing an order here

Anti-CCNB1 Polyclonal Antibody [PHD05701]
Terms of sale Website terms of use Cookie policy Privacy
Copyright © 2024 AntibodySystem SAS. All Rights Reserved.            All Products are for Research Use Only