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Anti-Human PGK2 Polyclonal Antibody

Catalog #:   PHC23901 Specific References (10) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P07205
Overview

Catalog No.

PHC23901

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PGK2 (Met1-Met417).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Phosphoglycerate kinase 2, PGKB, PGK2, Phosphoglycerate kinase, testis specific

Purification

Purified by antigen affinity column.

Accession

P07205

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with PGK2 antibody (PHC23901) at 1μg/ml.

    Lane 1: U87 cell lysate
    Lane 2: Jurkat cell lysate
    Lane 3: Hela cell lysate
    Lane 4: HepG2 cell lysate
    Lane 5: A549 cell lysate
    Lane 6: Raji cell lysate
    Lane 7: Molt-4 cell lysate
    Lane 8: Mouse brain lysate
    Lane 9: Mouse heart lysate
    Lane 10: Mouse testis lysate
    Lane 11: Rat brain lysate
    Lane 12: Rat heart lysate
    Lane 13: Rat testis lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 45 kDa
    Observed MW: 45 kDa
References

The Role of Chorein Deficiency in Late Spermatogenesis., PMID:38275411

Dietary quercetin and vitamin E supplementation modulates the reproductive performance and antioxidant capacity of aged male breeder chickens., PMID:35472738

Aberrant expression of sperm‑specific glycolytic enzymes are associated with poor sperm quality., PMID:30720094

Characteristics of testis-specific phosphoglycerate kinase 2 and its association with human sperm quality., PMID:26677959

Identification of genital tract markers in the human seminal plasma using an integrative genomics approach., PMID:23024119

Gonadotropin-regulated testicular RNA helicase (GRTH/Ddx25) is a transport protein involved in gene-specific mRNA export and protein translation during spermatogenesis., PMID:16968703

Direct effect of basic fibroblast growth factor on gene transcription in a cell-free system., PMID:1535155

Immunohistochemical localization of mouse testis-specific phosphoglycerate kinase (PGK-2) by monoclonal antibodies., PMID:6765230

Immunofluorescent localization of PGK-1 and PGK-2 isozymes within specific cells of the mouse testis., PMID:7026322

Immunological studies of the sperm-specific phosphoglycerate kinase-2 of mice., PMID:551176

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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Distributor list

For research use only. Not for human or drug use.

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Anti-Human PGK2 Polyclonal Antibody [PHC23901]
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