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Anti-Human PSMB5 Polyclonal Antibody

Catalog #:   PHD76901 Specific References (6) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P28074
Overview

Catalog No.

PHD76901

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PSMB5 (Gly59-Pro263).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

MB1, PSMB5, Proteasome subunit MB1, X, Macropain epsilon chain, Proteasome subunit beta type-5, Multicatalytic endopeptidase complex epsilon chain, LMPX, Proteasome subunit X, Proteasome chain 6, Proteasome epsilon chain

Purification

Purified by antigen affinity column.

Accession

P28074

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with PSMB5 antibody (PHD76901) at 1μg/ml.

    Lane 1: NIH3T3 cell lysate
    Lane 2: Hela cell lysate
    Lane 3: HepG2 cell lysate
    Lane 4: Jurkat cell lysate
    Lane 5: LO2 cell lysate
    Lane 6: U2OS cell lysate
    Lane 7: K562 cell lysate
    Lane 8: PC-3 cell lysate
    Lane 9: MCF-7 cell lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 28 kDa
    Observed MW: 22 kDa
References

Novel mechanism of drug resistance to proteasome inhibitors in multiple myeloma., PMID:31572666

Expression of possible targets for new proteasome inhibitors in diffuse large B-cell lymphoma., PMID:27301795

Immunoaffinity purification of the functional 20S proteasome from human cells via transient overexpression of specific proteasome subunits., PMID:24583181

Overcoming bortezomib resistance in human B cells by anti-CD20/rituximab-mediated complement-dependent cytotoxicity and epoxyketone-based irreversible proteasome inhibitors., PMID:23305345

Polyclonal antibodies against human proteasome subunits PSMA3, PSMA5, and PSMB5., PMID:22894781

[Comparison of protein expression profiles between bortezomib-resistant JurkatB cells with PSMB5 mutation and their parent cells]., PMID:21867604

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

Contact Information

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Distributor list

For research use only. Not for human or drug use.

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Anti-Human PSMB5 Polyclonal Antibody [PHD76901]
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