Catalog No.
PHA10501
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human WASL (Leu21-Arg148).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
N-WASP, WASL, Neural Wiskott-Aldrich syndrome protein
Purification
Purified by antigen affinity column.
Accession
O00401
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
N-WASP-dependent branched actin polymerization attenuates B-cell receptor signaling by increasing the molecular density of receptor clusters., PMID:38085658
WASP family proteins regulate the mobility of the B cell receptor during signaling activation., PMID:31974357
VCA nanobodies target N-WASp to reduce invadopodium formation and functioning., PMID:28938008
EtpE Binding to DNase X Induces Ehrlichial Entry via CD147 and hnRNP-K Recruitment, Followed by Mobilization of N-WASP and Actin., PMID:26530384
N-WASP is required for B-cell-mediated autoimmunity in Wiskott-Aldrich syndrome., PMID:26468226
Effect of omega-3 polyunsaturated fatty acids on the cytoskeleton: an open-label intervention study., PMID:25971815
Conditional N-WASP knockout in mouse brain implicates actin cytoskeleton regulation in hydrocephalus pathology., PMID:24462670
N-wasp is essential for the negative regulation of B cell receptor signaling., PMID:24223520
Serine phosphorylation of cortactin is required for maximal host cell invasion by Campylobacter jejuni., PMID:24188565
Recent advances in chronic lymphocytic leukemia., PMID:22842181
The WASp-based actin polymerization machinery is required in somatic support cells for spermatid maturation and release., PMID:21652648
The fadD2 gene is required for efficient Mycobacterium avium invasion of mucosal epithelial cells., PMID:16544254
The NF2 tumor suppressor Merlin and the ERM proteins interact with N-WASP and regulate its actin polymerization function., PMID:15699051
Cellular localization of activated N-WASP using a conformation-sensitive antibody., PMID:15362118
Inducible clustering of membrane-targeted SH3 domains of the adaptor protein Nck triggers localized actin polymerization., PMID:14711409
Impaired signaling via the high-affinity IgE receptor in Wiskott-Aldrich syndrome protein-deficient mast cells., PMID:14645152
Actin-based motility of Burkholderia pseudomallei involves the Arp 2/3 complex, but not N-WASP and Ena/VASP proteins., PMID:12780776
Phosphorylation of the WASP-VCA domain increases its affinity for the Arp2/3 complex and enhances actin polymerization by WASP., PMID:12769847
Neural Wiskott-Aldrich syndrome protein is recruited to rafts and associates with endophilin A in response to epidermal growth factor., PMID:12477732
Syndapins integrate N-WASP in receptor-mediated endocytosis., PMID:12426380
The F-actin side binding activity of the Arp2/3 complex is essential for actin nucleation and lamellipod extension., PMID:11369208
WIP regulates N-WASP-mediated actin polymerization and filopodium formation., PMID:11331876
N-WASP, WAVE and Mena play different roles in the organization of actin cytoskeleton in lamellipodia., PMID:11282031
Role of the PI3K regulatory subunit in the control of actin organization and cell migration., PMID:11038173
Activation by Cdc42 and PIP(2) of Wiskott-Aldrich syndrome protein (WASp) stimulates actin nucleation by Arp2/3 complex., PMID:10995437
A complex of N-WASP and WIP integrates signalling cascades that lead to actin polymerization., PMID:10878810
Syndapin I, a synaptic dynamin-binding protein that associates with the neural Wiskott-Aldrich syndrome protein., PMID:9950691
Inhibition of the development of spontaneous autoimmune thyroiditis in the obese strain (OS) chickens by in vivo treatment with anti-CD4 or anti-CD8 antibodies., PMID:9650090
Identification of N-WASP homologs in human and rat brain., PMID:9322739
Prevention of spontaneous autoimmune thyroiditis in the obese strain (OS) chickens by treatment with a monoclonal anti-CD4 antibody., PMID:8767730
For research use only. Not for human or drug use.
