Catalog No.
PHK51501
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human UPF1 (Val292-Gln710).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
hUpf1, Nonsense mRNA reducing factor 1, UPF1, Regulator of nonsense transcripts 1, RENT1, KIAA0221, ATP-dependent helicase RENT1, NORF1, Up-frameshift suppressor 1 homolog
Purification
Purified by antigen affinity column.
Accession
Q92900
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
UPF1 plays critical roles in early B cell development., PMID:38982067
The G allele of SNP rs3922 reduces the binding affinity between IGF2BP3 and CXCR5 correlating with a lower antibody production., PMID:37141498
Defining nonsense-mediated mRNA decay intermediates in human cells., PMID:30576707
Posttranscriptional Regulation of LOXL1 Expression Via Alternative Splicing and Nonsense-Mediated mRNA Decay as an Adaptive Stress Response., PMID:29164236
The nonsense-mediated RNA decay pathway is disrupted in inflammatory myofibroblastic tumors., PMID:27348585
Targeted Knock-Down of miR21 Primary Transcripts Using snoMEN Vectors Induces Apoptosis in Human Cancer Cell Lines., PMID:26405811
Cotranscriptional effect of a premature termination codon revealed by live-cell imaging., PMID:22028363
Incomplete nonsense-mediated mRNA decay in Giardia lamblia., PMID:18430426
Caenorhabditis elegans SMG-2 selectively marks mRNAs containing premature translation termination codons., PMID:17562857
Transcriptional silencing of nonsense codon-containing immunoglobulin micro genes requires translation of its mRNA., PMID:17428806
Alternative splicing induced by nonsense mutations in the immunoglobulin mu VDJ exon is independent of truncation of the open reading frame., PMID:15613538
Complexes between the nonsense-mediated mRNA decay pathway factor human upf1 (up-frameshift protein 1) and essential nonsense-mediated mRNA decay factors in HeLa cells., PMID:12723973
Identification of delta helicase as the bovine homolog of HUPF1: demonstration of an interaction with the third subunit of DNA polymerase delta., PMID:12000843
Identification and characterization of human orthologues to Saccharomyces cerevisiae Upf2 protein and Upf3 protein (Caenorhabditis elegans SMG-4)., PMID:11113196
SMG-2 is a phosphorylated protein required for mRNA surveillance in Caenorhabditis elegans and related to Upf1p of yeast., PMID:10454541
Cloning and characterization of HUPF1, a human homolog of the Saccharomyces cerevisiae nonsense mRNA-reducing UPF1 protein., PMID:9064659
For research use only. Not for human or drug use.
