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Anti-Human COG3 Polyclonal Antibody

Catalog #:   PHK50201 Specific References (6) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: Q96JB2
Overview

Catalog No.

PHK50201

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human COG3 (Met130-Leu274).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

COG complex subunit 3, Vesicle-docking protein SEC34 homolog, Component of oligomeric Golgi complex 3, COG3, Conserved oligomeric Golgi complex subunit 3, p94, SEC34

Purification

Purified by antigen affinity column.

Accession

Q96JB2

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN-BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with COG3 antibody (PHK50201) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 19 kDa
    Observed MW: 19 kDa
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with COG3 antibody (PHK50201) at 1 μg/ml.

    Lane 1: Hela

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 94 kDa
    Observed MW: 95 kDa
  • Immunohistochemical
    Immunohistochemical analysis of human prostate stained for COG3 with PHK50201.
References

Differential effects of lobe A and lobe B of the Conserved Oligomeric Golgi complex on the stability of {beta}1,4-galactosyltransferase 1 and {alpha}2,6-sialyltransferase 1., PMID:21062782

Rab6 regulates both ZW10/RINT-1 and conserved oligomeric Golgi complex-dependent Golgi trafficking and homeostasis., PMID:17699596

Retrograde transport of the mannosyltransferase Och1p to the early Golgi requires a component of the COG transport complex., PMID:15229219

Sec34 is implicated in traffic from the endoplasmic reticulum to the Golgi and exists in a complex with GTC-90 and ldlBp., PMID:11929878

Sgf1p, a new component of the Sec34p/Sec35p complex., PMID:11733049

Identification of a human orthologue of Sec34p as a component of the cis-Golgi vesicle tethering machinery., PMID:11292827

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

Contact Information

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Distributor list

For research use only. Not for human or drug use.

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Anti-Human COG3 Polyclonal Antibody [PHK50201]
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