Catalog No.
PHK50101
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human CARM1 (Thr28-Asn471).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
Histone-arginine methyltransferase CARM1, CARM1, Coactivator-associated arginine methyltransferase 1, PRMT4, Protein arginine N-methyltransferase 4
Purification
Purified by antigen affinity column.
Accession
Q86X55
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
XLID syndrome gene Med12 promotes Ig isotype switching through chromatin modification and enhancer RNA regulation., PMID:36427307
The circular RNA circHMGB2 drives immunosuppression and anti-PD-1 resistance in lung adenocarcinomas and squamous cell carcinomas via the miR-181a-5p/CARM1 axis., PMID:35525959
Targeting the IRE1α/XBP1s pathway suppresses CARM1-expressing ovarian cancer., PMID:34493732
A hypermethylation strategy utilized by enhancer-bound CARM1 to promote estrogen receptor α-dependent transcriptional activation and breast carcinogenesis., PMID:32206101
CARM1 methylates MED12 to regulate its RNA-binding ability., PMID:30456381
The co-activator-associated arginine methyltransferase 1 (CARM1) gene is overexpressed in type 2 diabetes., PMID:30173329
The Overexpression of CARM1 Promotes Human Osteosarcoma Cell Proliferation through the pGSK3β/β-Catenin/cyclinD1 Signaling Pathway., PMID:28924379
Using oriented peptide array libraries to evaluate methylarginine-specific antibodies and arginine methyltransferase substrate motifs., PMID:27338245
Subsets of Visceral Adipose Tissue Nuclei with Distinct Levels of 5-Hydroxymethylcytosine., PMID:27171244
Differential CARM1 Isoform Expression in Subcellular Compartments and among Malignant and Benign Breast Tumors., PMID:26030442
Mapping of Post-translational Modifications of Transition Proteins, TP1 and TP2, and Identification of Protein Arginine Methyltransferase 4 and Lysine Methyltransferase 7 as Methyltransferase for TP2., PMID:25818198
CARM1 automethylation is controlled at the level of alternative splicing., PMID:23723242
A TR-FRET-based functional assay for screening activators of CARM1., PMID:23585185
Methods applied to the study of protein arginine methylation., PMID:22910203
HuD interacts with survival motor neuron protein and can rescue spinal muscular atrophy-like neuronal defects., PMID:21088113
Regulation of post-translational protein arginine methylation during HeLa cell cycle., PMID:20541591
Biochemical analysis of arginine methylation in transcription., PMID:19381935
The arginine methyltransferase CARM1 regulates the coupling of transcription and mRNA processing., PMID:17218272
The activity and stability of the transcriptional coactivator p/CIP/SRC-3 are regulated by CARM1-dependent methylation., PMID:17043108
Coactivator-associated arginine methyltransferase-1 enhances nuclear factor-kappaB-mediated gene transcription through methylation of histone H3 at arginine 17., PMID:16497732
Lipopolysaccharide-induced methylation of HuR, an mRNA-stabilizing protein, by CARM1. Coactivator-associated arginine methyltransferase., PMID:12237300
Methylation at arginine 17 of histone H3 is linked to gene activation., PMID:11751582
Hormone-dependent, CARM1-directed, arginine-specific methylation of histone H3 on a steroid-regulated promoter., PMID:11747826
Methylation of histone H4 at arginine 3 occurs in vivo and is mediated by the nuclear receptor coactivator PRMT1., PMID:11448779
For research use only. Not for human or drug use.
