Overview
Catalog No.
PHA13801
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human PSMD14 (Gln30-Lys209).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
26S proteasome non-ATPase regulatory subunit 14, 26S proteasome-associated PAD1 homolog 1, POH1, 26S proteasome regulatory subunit RPN11, PSMD14
Purification
Purified by antigen affinity column.
Accession
O00487
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Data Image
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Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with PSMD14 antibody (PHA13801) at 1 μg/ml.
Lane 1: Recombinant protein
Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.
Predict MW: 22 kDa
Observed MW: 22 kDa
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Various lysates were subjected to SDS PAGE followed by western blot with PSMD14 antibody (PHA13801) at 1 μg/ml.
Lane 1: COS7
Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.
Predict MW: 35 kDa
Observed MW: 35 kDa
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Immunohistochemical analysis of rat ovary tissue stained for PSMD14 with PHA13801.
References
ALKBH1 knockdown promotes the growth, migration and invasion of HTR-8/SVneo cells through regulating the m5C modification PSMD14., PMID:40025166
Alterations in the expression pattern of RBC membrane associated proteins (RMAPs) in whole body γ-irradiated Sprague Dawley rats., PMID:37315317
Sequential ubiquitination and deubiquitination enzymes synchronize the dual sensor and effector functions of TRIM21., PMID:26150489
Crystal structure of the proteasomal deubiquitylation module Rpn8-Rpn11., PMID:24516147
Co-operation of BRCA1 and POH1 relieves the barriers posed by 53BP1 and RAP80 to resection., PMID:24013561
Regulation of ErbB2 receptor status by the proteasomal DUB POH1., PMID:19436748
Datasheet
For research use only. Not for human or drug use.
