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Anti-Human PSMB1 Polyclonal Antibody

Catalog #:   PHD40401 Specific References (8) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P20618
Overview

Catalog No.

PHD40401

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PSMB1 (Gly38-Asp241).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

PSMB1, Macropain subunit C5, Multicatalytic endopeptidase complex subunit C5, Proteasome gamma chain, Proteasome subunit beta type-1, PSC5, Proteasome component C5

Purification

Purified by antigen affinity column.

Accession

P20618

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with PSMB1 antibody (PHD40401) at 1μg/ml.

    Lane 1: Hela cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: MCF-7 cell lysate
    Lane 4: K562 cell lysate
    Lane 5: THP-1 cell lysate
    Lane 6: U937 cell lysate
    Lane 7: Mouse spleen lysate
    Lane 8: Mouse liver lysate
    Lane 9: Rat spleen lysate
    Lane 10: Rat liver lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 26 kDa
    Observed MW: 25 kDa
  • Immunohistochemical
    Immunohistochemical analysis of rat brain tissue stained for PSMB1 with PHD40401.
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with PSMB1 antibody (PHD40401) at 1 µg/ml.

    Lane 1: Recombinant Protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 25 kDa
    Observed MW: 25 kDa
References

Increased peptidylarginine deiminases expression during the macrophage differentiation and participated inflammatory responses., PMID:31039829

Predictive biomarkers may help individualize treatment for patients with follicular lymphoma., PMID:23842891

Prespecified candidate biomarkers identify follicular lymphoma patients who achieved longer progression-free survival with bortezomib-rituximab versus rituximab., PMID:23549871

The role of proteasome beta subunits in gastrin-mediated transcription of plasminogen activator inhibitor-2 and regenerating protein1., PMID:23544109

Polyclonal antibodies against human proteasome subunits PSMA3, PSMA5, and PSMB5., PMID:22894781

BCL-3 degradation involves its polyubiquitination through a FBW7-independent pathway and its binding to the proteasome subunit PSMB1., PMID:20558726

Identification of a gastrin response element in the vesicular monoamine transporter type 2 promoter and requirement of 20 S proteasome subunits for transcriptional activity., PMID:17442673

Intermediates in the formation of mouse 20S proteasomes: implications for the assembly of precursor beta subunits., PMID:9311996

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-Human PSMB1 Polyclonal Antibody [PHD40401]
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