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Anti-Human MMP28 Polyclonal Antibody

Catalog #:   PHJ55201 Specific References (7) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: Q9H239
Overview

Catalog No.

PHJ55201

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human MMP28 (Lys322-Phe520).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

MMP-28, Epilysin, MMP28, MMP25, Matrix metalloproteinase-28

Purification

Purified by antigen affinity column.

Accession

Q9H239

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN-BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with MMP28 antibody (PHJ55201) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 25 kDa
    Observed MW: 25 kDa
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with MMP28 antibody (PHJ55201) at 1 μg/ml.

    Lane 1: Hela
    Lane 2: MCF7

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 59 kDa
    Observed MW: 59 kDa
  • Immunohistochemical
    Immunohistochemical analysis of human pancreas stained for MMP28 with PHJ55201.
References

Infliximab restores the dysfunctional matrix remodeling protein and growth factor gene expression in patients with inflammatory bowel disease., PMID:24378596

Nicotine modulates gelatinase B (MMP-9) and epilysin (MMP-28) expression in reconstituted human oral epithelium., PMID:20923448

Epilysin (MMP-28) is deposited to the basolateral extracellular matrix of epithelial cells., PMID:19379669

MMP-28 as a regulator of myelination., PMID:18778487

Epilysin (MMP-28) induces TGF-beta mediated epithelial to mesenchymal transition in lung carcinoma cells., PMID:16940349

Differential expression of three matrix metalloproteinases, MMP-19, MMP-26, and MMP-28, in normal and inflamed intestine and colon cancer., PMID:15185874

Promoter characterization of the human and mouse epilysin (MMP-28) genes., PMID:11574168

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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Distributor list

For research use only. Not for human or drug use.

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Anti-Human MMP28 Polyclonal Antibody [PHJ55201]
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