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Anti-Zaire ebolavirus NP/Nucleoprotein Polyclonal Antibody

Catalog #:   PVV22701 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: AXF48955.1
Overview

Catalog No.

PVV22701

Species reactivity

Zaire ebolavirus

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Zaire ebolavirus NP/Nucleo(Val36-Glu351).

Tested applications

ELISA: 1:4000-1:8000, WB: 1:1000-1:4000

Target

Nucleoprotein, Nucleocapsid protein, NP

Purification

Purified by antigen affinity column.

Accession

AXF48955.1

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Recombinant Protein were subjected to SDS PAGE followed by western blot with ebola_NP antibody (PVV22701) at 1μg/ml.

    Lane 1: : Recombinant Zaire ebolavirus NP/Nucleoprotein Protein(YVV22701)

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 34 kDa
    Observed MW: 34 kDa
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with NP/Nucleoprotein antibody (PVV22701) at 1 µg/ml.

    Lane 1: Recombinant Protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 36 kDa
    Observed MW: 36 kDa
References

Ebola and Marburg viruses IgG detections in small ruminants and dogs from villages within outbreak areas in Gabon., PMID:40043032

Serological evidence of zoonotic filovirus exposure among bushmeat hunters in Guinea., PMID:38755147

Molecular modeling and simulation studies of SELEX-derived high-affinity DNA aptamers to the Ebola virus nucleoprotein., PMID:38217874

Low seroprevalence of Ebola virus in health care providers in an endemic region (Tshuapa province) of the Democratic Republic of the Congo., PMID:37656725

Diagnostics of Ebola virus., PMID:36908455

Cellular and humoral immunity to Ebola Zaire glycoprotein and viral vector proteins following immunization with recombinant vesicular stomatitis virus-based Ebola vaccine (rVSVΔG-ZEBOV-GP)., PMID:36725433

Randomized Trial of Vaccines for Zaire Ebola Virus Disease., PMID:36516078

Self-amplifying RNA vaccine protects mice against lethal Ebola virus infection., PMID:36303436

Detection of filovirus-reactive antibodies in Australian bat species., PMID:35972225

Ebola-Detect: A differential serodiagnostic assay for Ebola virus infections and surveillance in the presence of vaccine-induced antibodies., PMID:35901660

Epitope-focused immunogen design based on the ebolavirus glycoprotein HR2-MPER region., PMID:35584193

Visualizing Filoviral Nucleoproteins Using Nanobodies Fused to the Ascorbate Peroxidase Derivatives APEX2 and dEAPX., PMID:35157287

Generating Uniformly Cross-Reactive Ebolavirus spp. Anti-nucleoprotein Nanobodies to Facilitate Forward Capable Detection Strategies., PMID:34994194

A computational overview on phylogenetic characterization, pathogenic mutations, and drug targets for Ebola virus disease., PMID:34563987

Investigation of Ebolavirus exposure in pigs presented for slaughter in Uganda., PMID:32915496

Characteristics of Ebola Virus Disease Survivor Blood and Semen in Liberia: Serology and Reverse Transcription Polymerase Chain Reaction (RT-PCR)., PMID:32894277

Identification of interferon-stimulated genes that attenuate Ebola virus infection., PMID:32528005

An evolutionary insight into emerging Ebolavirus strains isolated in Africa., PMID:31702053

Serological evidence of exposure to ebolaviruses in domestic pigs from Guinea., PMID:31627257

Paratope Duality and Gullying are Among the Atypical Recognition Mechanisms Used by a Trio of Nanobodies to Differentiate Ebolavirus Nucleoproteins., PMID:31626803

Serological analysis of Ebola virus survivors and close contacts in Sierra Leone: A cross-sectional study., PMID:31369554

Evaluation of Diagnostic Performance of Three Indirect Enzyme-Linked Immunosorbent Assays for the Detection of IgG Antibodies to Ebola Virus in Human Sera., PMID:31344850

Multiple viral proteins and immune response pathways act to generate robust long-term immunity in Sudan virus survivors., PMID:31326432

Defining the multiplicity and time of infection for the production of Zaire Ebola virus-like particles in the insect cell-baculovirus expression system., PMID:31262589

Periplasmic Nanobody-APEX2 Fusions Enable Facile Visualization of Ebola, Marburg, and Mĕnglà virus Nucleoproteins, Alluding to Similar Antigenic Landscapes among Marburgvirus and Dianlovirus., PMID:31010013

Generation and Characterization of Anti-Filovirus Nucleoprotein Monoclonal Antibodies., PMID:30875741

Naturally Occurring Single Mutations in Ebola Virus Observably Impact Infectivity., PMID:30333174

Pan-Filovirus Serum Neutralizing Antibodies in a Subset of Congolese Ebolavirus Infection Survivors., PMID:30107445

Analysis of CD8+ T cell response during the 2013-2016 Ebola epidemic in West Africa., PMID:30038008

Unveiling a Drift Resistant Cryptotope within Marburgvirus Nucleoprotein Recognized by Llama Single-Domain Antibodies., PMID:29038656

Intracellular Crosslinking of Filoviral Nucleoproteins with Xintrabodies Restricts Viral Packaging., PMID:29021793

Dynamic phosphorylation of Ebola virus VP30 in NP-induced inclusion bodies., PMID:28915404

Recombinant Modified Vaccinia Virus Ankara Generating Ebola Virus-Like Particles., PMID:28331098

Attenuated Human Parainfluenza Virus Type 1 Expressing Ebola Virus Glycoprotein GP Administered Intranasally Is Immunogenic in African Green Monkeys., PMID:28250127

Production, purification and immunogenicity of recombinant Ebola virus proteins - A comparison of Freund's adjuvant and adjuvant system 03., PMID:28025125

Rapid detection of viral antibodies based on multifunctional Staphylococcus aureus nanobioprobes., PMID:27866631

Development of a Sensitive and Specific Serological Assay Based on Luminex Technology for Detection of Antibodies to Zaire Ebola Virus., PMID:27795350

The Ebola Virus VP30-NP Interaction Is a Regulator of Viral RNA Synthesis., PMID:27755595

An Ebola Virus-Like Particle-Based Reporter System Enables Evaluation of Antiviral Drugs In Vivo under Non-Biosafety Level 4 Conditions., PMID:27440895

Human Survivors of Disease Outbreaks Caused by Ebola or Marburg Virus Exhibit Cross-Reactive and Long-Lived Antibody Responses., PMID:27335383

Determination of specific antibody responses to the six species of ebola and Marburg viruses by multiplexed protein microarrays., PMID:25230936

Identification of continuous human B-cell epitopes in the VP35, VP40, nucleoprotein and glycoprotein of Ebola virus., PMID:24914933

Mapping of conserved and species-specific antibody epitopes on the Ebola virus nucleoprotein., PMID:23702199

Profile and persistence of the virus-specific neutralizing humoral immune response in human survivors of Sudan ebolavirus (Gulu)., PMID:23585686

Ebolavirus nucleoprotein C-termini potently attract single domain antibodies enabling monoclonal affinity reagent sandwich assay (MARSA) formulation., PMID:23577211

Generation and epitope mapping of a monoclonal antibody against nucleoprotein of Ebola virus., PMID:23457784

Analysis of the humoral immune responses among cynomolgus macaque naturally infected with Reston virus during the 1996 outbreak in the Philippines., PMID:23057674

Profiling the native specific human humoral immune response to Sudan Ebola virus strain Gulu by chemiluminescence enzyme-linked immunosorbent assay., PMID:22993411

sGP serves as a structural protein in Ebola virus infection., PMID:21987767

A replicating cytomegalovirus-based vaccine encoding a single Ebola virus nucleoprotein CTL epitope confers protection against Ebola virus., PMID:21858240

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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Anti-Zaire ebolavirus NP/Nucleoprotein Polyclonal Antibody [PVV22701]
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