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Anti-Human RAP1B Polyclonal Antibody

Catalog #:   PHF41401 Specific References (44) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P61224
Overview

Catalog No.

PHF41401

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human RAP1B (Met1-Lys168).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Ras-related protein Rap-1b, RAP1B, GTP-binding protein smg p21B

Purification

Purified by antigen affinity column.

Accession

P61224

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN-BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with RAP1B antibody (PHF41401) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 21 kDa
    Observed MW: 21 kDa
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with RAP1B antibody (PHF41401) at 1 μg/ml.

    Lane 1: Mouse brain
    Lane 2: A549
    Lane 3: A431

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 21 kDa
    Observed MW: 21 kDa
  • Immunohistochemical
    Immunohistochemical analysis of human lung cancer stained for RAP1B with PHF41401.
References

miR-28-5p targeted Rap1b attenuates splenic inflammation infiltration in immune thrombocytopenia., PMID:40197264

H3K36 methyltransferase NSD1 is essential for normal B1 and B2 cell development and germinal center formation., PMID:36532012

Theileria equi RAP-1a and RAP-1b proteins contain immunoreactive epitopes and are suitable candidates for vaccine and diagnostics development., PMID:35318949

The proteomes of endometrial stromal cell-derived extracellular vesicles following a decidualizing stimulus define the cells' potential for decidualization success., PMID:34524461

Rap1 Is Essential for B-Cell Locomotion, Germinal Center Formation and Normal B-1a Cell Population., PMID:34140948

Extracellular Vesicle and Particle Biomarkers Define Multiple Human Cancers., PMID:32795414

Large Extracellular Vesicle-Associated Rap1 Accumulates in Atherosclerotic Plaques, Correlates With Vascular Risks and Is Involved in Atherosclerosis., PMID:32539601

Two novel, putative mechanisms of action for citalopram-induced platelet inhibition., PMID:30420683

The Rap1-cofilin-1 pathway coordinates actin reorganization and MTOC polarization at the B cell immune synapse., PMID:28167682

RAP-1a is the main rhoptry-associated-protein-1 (RAP-1) recognized during infection with Babesia sp. BQ1 (Lintan) (B. motasi-like phylogenetic group), a pathogen of sheep in China., PMID:27890082

GABAB receptor promotes its own surface expression by recruiting a Rap1-dependent signaling cascade., PMID:25967549

Glucocorticoids mediate induction of microRNA-708 to suppress ovarian cancer metastasis through targeting Rap1B., PMID:25569036

The Ras-related protein, Rap1A, mediates thrombin-stimulated, integrin-dependent glioblastoma cell proliferation and tumor growth., PMID:24790104

Phosphorylation of CalDAG-GEFI by protein kinase A prevents Rap1b activation., PMID:23611601

A novel neutralization sensitive and subdominant RAP-1-related antigen (RRA) is expressed by Babesia bovis merozoites., PMID:21554842

Statins inhibit T-acute lymphoblastic leukemia cell adhesion and migration through Rap1b., PMID:21233409

Junctional adhesion molecule A interacts with Afadin and PDZ-GEF2 to activate Rap1A, regulate beta1 integrin levels, and enhance cell migration., PMID:19176753

Rap1b regulates B cell development, homing, and T cell-dependent humoral immunity., PMID:18714009

Critical role of ADP interaction with P2Y12 receptor in the maintenance of alpha(IIb)beta3 activation: association with Rap1B activation., PMID:16706985

Identification and biochemical characterization of Rap2C, a new member of the Rap family of small GTP-binding proteins., PMID:16213650

Thrombopoietin complements G(i)- but not G(q)-dependent pathways for integrin {alpha}(IIb){beta}(3) activation and platelet aggregation., PMID:15863506

The sequential activity of the GTPases Rap1B and Cdc42 determines neuronal polarity., PMID:15286792

Contribution of protease-activated receptors 1 and 4 and glycoprotein Ib-IX-V in the G(i)-independent activation of platelet Rap1B by thrombin., PMID:15078882

High glucose stimulates synthesis of fibronectin via a novel protein kinase C, Rap1b, and B-Raf signaling pathway., PMID:12196513

Relationships between Rap1b, affinity modulation of integrin alpha IIbbeta 3, and the actin cytoskeleton., PMID:11994301

Identification of up-regulated Ras-like GTPase, Rap1b, by suppression subtractive hybridization., PMID:11737587

Rap1B and Rap2B translocation to the cytoskeleton by von Willebrand factor involves FcgammaII receptor-mediated protein tyrosine phosphorylation., PMID:10224142

Disruption of the mouse Rce1 gene results in defective Ras processing and mislocalization of Ras within cells., PMID:10085069

Expression and localization of RAP1 proteins during myogenic differentiation., PMID:8900487

Alpha-granule membrane mirrors the platelet plasma membrane and contains the glycoproteins Ib, IX, and V., PMID:8608228

Activation of brain B-Raf protein kinase by Rap1B small GTP-binding protein., PMID:8576107

Ultrastructural localization of the small GTP-binding protein Rap1 in human platelets and megakaryocytes., PMID:7803284

Association of Rap1a and Rap1b proteins with late endocytic/phagocytic compartments and Rap2a with the Golgi complex., PMID:7962206

Evidence for Rap1 in vascular smooth muscle cells. Regulation of their expression by platelet-derived growth factor BB., PMID:8143870

Correlated expression of the 97 kDa sarcoendoplasmic reticulum Ca(2+)-ATPase and Rap1B in platelets and various cell lines., PMID:8297341

Purification of a novel ras GTPase-activating protein from rat brain., PMID:8226805

Mutational analysis of the cAMP-dependent protein kinase-mediated phosphorylation site of Rap1b., PMID:8463283

Role of rap1B and p21ras GTPase-activating protein in the regulation of phospholipase C-gamma 1 in human platelets., PMID:1323853

Generation of specific antibodies against the rap1A, rap1B and rap2 small GTP-binding proteins. Analysis of rap and ras proteins in membranes from mammalian cells., PMID:1628649

Rap1A is a substrate for cyclic AMP-dependent protein kinase in human neutrophils., PMID:1908879

Association of the Ras-antagonistic Rap1/Krev-1 proteins with the Golgi complex., PMID:1900364

RAP2B: a RAS-related GTP-binding protein from platelets., PMID:2118648

Rap1-B is phosphorylated by protein kinase A in intact human platelets., PMID:1696481

Tissue and subcellular distributions of the smg-21/rap1/Krev-1 proteins which are partly distinct from those of c-ras p21s., PMID:2111441

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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Anti-Human RAP1B Polyclonal Antibody [PHF41401]
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