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Anti-Human RAB12 Polyclonal Antibody

Catalog #:   PHK48301 Specific References (7) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: Q6IQ22
Overview

Catalog No.

PHK48301

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human RAB12 (Asp40-Lys207).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

RAB12, Ras-related protein Rab-12

Purification

Purified by antigen affinity column.

Accession

Q6IQ22

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN-BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with RAB12 antibody (PHK48301) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 22 kDa
    Observed MW: 22 kDa
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with RAB12 antibody (PHK48301) at 1 μg/ml.

    Lane 1: Rat cerebellum
    Lane 2: Rat heart

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 27 kDa
    Observed MW: 27 kDa
  • Immunohistochemical
    Immunohistochemical analysis of human kidney tissue stained for RAB12 with PHK48301.
References

Preclinical modeling of chronic inhibition of the Parkinson's disease associated kinase LRRK2 reveals altered function of the endolysosomal system in vivo., PMID:33741046

The Parkinson's disease VPS35[D620N] mutation enhances LRRK2-mediated Rab protein phosphorylation in mouse and human., PMID:29743203

Development of phospho-specific Rab protein antibodies to monitor in vivo activity of the LRRK2 Parkinson's disease kinase., PMID:29127256

Identification and characterisation of novel Mss4-binding Rab GTPases., PMID:21194374

Immunoprecipitation and MALDI-MS identification of lithocholic acid-tagged proteins in liver of bile duct-ligated rats., PMID:18641422

Identification of rab12 as a secretory granule-associated small GTP-binding protein in atrial myocytes., PMID:8575079

Molecular cloning and subcellular localization of three GTP-binding proteins of the rab subfamily., PMID:8126105

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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Distributor list

For research use only. Not for human or drug use.

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Anti-Human RAB12 Polyclonal Antibody [PHK48301]
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