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Anti-Human UFD1 Polyclonal Antibody

Catalog #:   PHK46901 Specific References (7) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: Q92890
Overview

Catalog No.

PHK46901

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human UFD1 (Arg18-Asp192).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

UFD1L, Ubiquitin recognition factor in ER-associated degradation protein 1, UB fusion protein 1, UFD1, Ubiquitin fusion degradation protein 1

Purification

Purified by antigen affinity column.

Accession

Q92890

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN-BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with UFD1 antibody (PHK46901) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 22 kDa
    Observed MW: 22 kDa
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with UFD1 antibody (PHK46901) at 1 μg/ml.

    Lane 1: HepG2
    Lane 2: Raji
    Lane 3: MCF7
    Lane 4: Jurkat

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 35 kDa
    Observed MW: 35 kDa
  • Immunohistochemical
    Immunohistochemical analysis of mouse kidney stained for UFD1 with PHK46901.
References

TRIM55 promotes noncanonical NF-κB signaling and B cell-mediated immune responses by coordinating p100 ubiquitination and processing., PMID:37816088

Phospho-Ser784-VCP Is Required for DNA Damage Response and Is Associated with Poor Prognosis of Chemotherapy-Treated Breast Cancer., PMID:32521270

Serum biomarkers in patients suspected of transient ischaemic attack in primary care: a diagnostic accuracy study., PMID:31628130

Cdc48/p97-Ufd1-Npl4 antagonizes Aurora B during chromosome segregation in HeLa cells., PMID:21486945

Analysis of Npl4 deletion mutants in mammalian cells unravels new Ufd1-interacting motifs and suggests a regulatory role of Npl4 in ERAD., PMID:18586029

Analysis of intracellular distribution and apoptosis involvement of the Ufd1l gene product by over-expression studies., PMID:12910480

Structure and expression of the human ubiquitin fusion-degradation gene (UFD1L)., PMID:9540831

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

Contact Information

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Distributor list

For research use only. Not for human or drug use.

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Anti-Human UFD1 Polyclonal Antibody [PHK46901]
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