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Anti-Human RAB14 Polyclonal Antibody

Catalog #:   PHF40401 Specific References (12) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P61106
Overview

Catalog No.

PHF40401

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human RAB14 (Met1-Ser180).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

RAB14, Ras-related protein Rab-14

Purification

Purified by antigen affinity column.

Accession

P61106

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN-BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with RAB14 antibody (PHF40401) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 22 kDa
    Observed MW: 22 kDa
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with RAB14 antibody (PHF40401) at 1 μg/ml.

    Lane 1: RH-30

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 24 kDa
    Observed MW: 24 kDa
  • Immunohistochemical
    Immunohistochemical analysis of human testis tissue stained for RAB14 with PHF40401.
References

Comprehensive knockout analysis of the RAB family small GTPases reveals an overlapping role of RAB2 and RAB14 in autophagosome maturation., PMID:38953305

Rab2 and Rab6 are Implicated in Acrosome Formation during Spermatogenesis in Eriocheir sinensis: Based on Sperm Proteome., PMID:37664930

Endocytosed HIV-1 Envelope Glycoprotein Traffics to Rab14+ Late Endosomes and Lysosomes to Regulate Surface Levels in T-Cell Lines., PMID:35770989

Acquisition of Rab11 and Rab11-Fip2-A novel strategy for Chlamydia pneumoniae early survival., PMID:28787457

Rab antibody characterization: comparison of Rab14 antibodies., PMID:25800840

Functional analysis of novel Rab GTPases identified in the proteome of purified Legionella-containing vacuoles from macrophages., PMID:24373249

Drosophila Rab14 mediates phagocytosis in the immune response to Staphylococcus aureus., PMID:24119134

Relationship between the expression of Rab family GTPases and neuropeptide hormones in the brain of Bombyx mori., PMID:22922733

Class I Rab11-family interacting proteins are binding targets for the Rab14 GTPase., PMID:19702578

Proteome analysis of Legionella vacuoles purified by magnetic immunoseparation reveals secretory and endosomal GTPases., PMID:18980612

Rab14 is involved in membrane trafficking between the Golgi complex and endosomes., PMID:15004230

The phagosome proteome: insight into phagosome functions., PMID:11149929

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

Contact Information

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Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

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Anti-Human RAB14 Polyclonal Antibody [PHF40401]
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