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Anti-Human GSPT1 Polyclonal Antibody

Catalog #:   PHD09301 Specific References (13) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P15170
Overview

Catalog No.

PHD09301

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human GSPT1 (Lys71-Asp499).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Eukaryotic peptide chain release factor subunit 3a, GSPT1, Eukaryotic peptide chain release factor GTP-binding subunit ERF3A, G1 to S phase transition protein 1 homolog, eRF3a, ERF3A

Purification

Purified by antigen affinity column.

Accession

P15170

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN-BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with GSPT1 antibody (PHD09301) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 50 kDa
    Observed MW: 50 kDa
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with GSPT1 antibody (PHD09301) at 1 μg/ml.

    Lane 1: A549
    Lane 2: HEK293T
    Lane 3: Hela

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 56 kDa
    Observed MW: 75 kDa
  • Immunohistochemical
    Immunohistochemical analysis of mouse stomach tissue stained for GSPT1 with PHD09301.
References

Comparative analysis of biophysical methods for monitoring protein proximity induction in the development of small molecule degraders., PMID:37301332

Vimentin binds to a novel tumor suppressor protein, GSPT1-238aa, encoded by circGSPT1 with a selective encoding priority to halt autophagy in gastric carcinoma., PMID:35839920

Recombinase-mediated cassette exchange-based screening of a CRISPR/Cas9 library for enhanced recombinant protein production in human embryonic kidney cells: Improving resistance to hyperosmotic stress., PMID:35398513

Comprehensive exploratory autoantibody profiling in patients with early rheumatoid arthritis treated with methotrexate or tocilizumab., PMID:33301475

Nicotine-mediated invasion and migration of non-small cell lung carcinoma cells by modulating STMN3 and GSPT1 genes in an ID1-dependent manner., PMID:25028095

Monoclonal antibodies against human translation termination factor eRF3 and their utilization for sub-cellular localization of eRF3., PMID:21421683

Nuclear localization of eukaryotic class II release factor (eRF3): implication for the multifunction of eRF3 in ciliates Euplotes cell., PMID:18296078

Newer potential biomarkers in prostate cancer., PMID:18231617

Expression, characterization and immunolocalization of translation termination factor eRF3 in the ciliate Euplotes octocarinatus., PMID:16229990

Depletion of a Drosophila homolog of yeast Sup35p disrupts spindle assembly, chromosome segregation, and cytokinesis during male meiosis., PMID:9556329

Immunochemical determination of the cellular content of polypeptide chain release factor RF3 in Escherichia coli., PMID:9523014

Comparative characterization of release factor RF-3 genes of Escherichia coli, Salmonella typhimurium, and Dichelobacter nodosus., PMID:7559341

A gene region in Dichelobacter nodosus encoding a lipopolysaccharide epitope., PMID:7539688

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

Contact Information

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Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

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Anti-Human GSPT1 Polyclonal Antibody [PHD09301]
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