Catalog No.
PHD09301
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human GSPT1 (Lys71-Asp499).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
Eukaryotic peptide chain release factor subunit 3a, GSPT1, Eukaryotic peptide chain release factor GTP-binding subunit ERF3A, G1 to S phase transition protein 1 homolog, eRF3a, ERF3A
Purification
Purified by antigen affinity column.
Accession
P15170
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Comparative analysis of biophysical methods for monitoring protein proximity induction in the development of small molecule degraders., PMID:37301332
Vimentin binds to a novel tumor suppressor protein, GSPT1-238aa, encoded by circGSPT1 with a selective encoding priority to halt autophagy in gastric carcinoma., PMID:35839920
Recombinase-mediated cassette exchange-based screening of a CRISPR/Cas9 library for enhanced recombinant protein production in human embryonic kidney cells: Improving resistance to hyperosmotic stress., PMID:35398513
Comprehensive exploratory autoantibody profiling in patients with early rheumatoid arthritis treated with methotrexate or tocilizumab., PMID:33301475
Nicotine-mediated invasion and migration of non-small cell lung carcinoma cells by modulating STMN3 and GSPT1 genes in an ID1-dependent manner., PMID:25028095
Monoclonal antibodies against human translation termination factor eRF3 and their utilization for sub-cellular localization of eRF3., PMID:21421683
Nuclear localization of eukaryotic class II release factor (eRF3): implication for the multifunction of eRF3 in ciliates Euplotes cell., PMID:18296078
Newer potential biomarkers in prostate cancer., PMID:18231617
Expression, characterization and immunolocalization of translation termination factor eRF3 in the ciliate Euplotes octocarinatus., PMID:16229990
Depletion of a Drosophila homolog of yeast Sup35p disrupts spindle assembly, chromosome segregation, and cytokinesis during male meiosis., PMID:9556329
Immunochemical determination of the cellular content of polypeptide chain release factor RF3 in Escherichia coli., PMID:9523014
Comparative characterization of release factor RF-3 genes of Escherichia coli, Salmonella typhimurium, and Dichelobacter nodosus., PMID:7559341
A gene region in Dichelobacter nodosus encoding a lipopolysaccharide epitope., PMID:7539688