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Anti-Human TRIM27 Polyclonal Antibody

Catalog #:   PHD04301 Specific References (12) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P14373
Overview

Catalog No.

PHD04301

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human TRIM27 (Ser315-Pro513).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

RING finger protein 76, Zinc finger protein RFP, RFP, TRIM27, RNF76, RING-type E3 ubiquitin transferase TRIM27, Tripartite motif-containing protein 27, Ret finger protein

Purification

Purified by antigen affinity column.

Accession

P14373

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN-BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with TRIM27 antibody (PHD04301) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 24 kDa
    Observed MW: 24 kDa
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with TRIM27 antibody (PHD04301) at 1 μg/ml.

    Lane 1: Jurkat
    Lane 2: HepG2

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 58 kDa
    Observed MW: 58 kDa
  • Immunohistochemical
    Immunohistochemical analysis of rat ovary tissue stained for TRIM27 with PHD04301.
References

The Myoepithelial Cells of Salivary Intercalated Duct-type Intraductal Carcinoma Are Neoplastic: A Study Using Combined Whole-slide Imaging, Immunofluorescence, and RET Fluorescence In Situ Hybridization., PMID:33086236

The identification of loci for immune traits in chickens using a genome-wide association study., PMID:25822738

Genome-wide association study of antibody level response to NDV and IBV in Jinghai yellow chicken based on SLAF-seq technology., PMID:25588649

Phosphatidylinositol-3-kinase C2β and TRIM27 function to positively and negatively regulate IgE receptor activation of mast cells., PMID:22645315

Sonic hedgehog expression in corticofugal projection neurons directs cortical microcircuit formation., PMID:22445340

Expression of Ret finger protein correlates with outcomes in endometrial cancer., PMID:19650860

Microinjection into cultured hippocampal neurons: a straightforward approach for controlled cellular delivery of nucleic acids, peptides and antibodies., PMID:18761372

Involvement of GDNF and its receptors in the maturation of the periodontal Ruffini endings., PMID:17178438

Interaction between the Ret finger protein and the Int-6 gene product and co-localisation into nuclear bodies., PMID:10504338

Involvement of the rfp tripartite motif in protein-protein interactions and subcellular distribution., PMID:9247190

RFP is a DNA binding protein associated with the nuclear matrix., PMID:1437549

Nuclear localization of antigens detected by a monoclonal antibody against a synthetic peptide of rfp finger protein., PMID:2112113

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

Contact Information

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Distributor list

For research use only. Not for human or drug use.

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Anti-Human TRIM27 Polyclonal Antibody [PHD04301]
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