Catalog No.
PHK45001
Species reactivity
Human, Mouse
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human ROBO1 (Arg69-Ala166).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
H-Robo-1, DUTT1, Roundabout homolog 1, Deleted in U twenty twenty, ROBO1
Purification
Purified by antigen affinity column.
Accession
Q9Y6N7
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Oculomotor axons use external and autocrine Slit signals for fasciculation, navigation, branching, and connectivity in mouse embryos., PMID:40470230
Expression Profiles of Five Common Cancer Membrane Protein Antigens Collected for the Development of Cocktail CAR-T Cell Therapies Applicable to Most Solid Cancer Patients., PMID:40076777
Monoclonal antibodies that block Roundabout 1 and 2 signaling target pathological ocular neovascularization through myeloid cells., PMID:39565875
TSHR-IGF-IR complex drives orbital fibroblast misbehavior in thyroid eye disease., PMID:39082947
Cetuximab-Toxin Conjugate and NPe6 with Light Enhanced Cytotoxic Effects in Head and Neck Squamous Cell Carcinoma In Vitro., PMID:38790935
Stromal Cell-SLIT3/Cardiomyocyte-ROBO1 Axis Regulates Pressure Overload-Induced Cardiac Hypertrophy., PMID:38414132
The tyrosine kinase inhibitor nintedanib enhances the efficacy of 90 Y-labeled B5209B radioimmunotherapy targeting ROBO1 without increased toxicity in small-cell lung cancer xenograft mice., PMID:37728607
Novel concept of Wilms' tumor development: involvement of pluripotential cells of ureteric bud., PMID:37209922
Coadaptation fostered by the SLIT2-ROBO1 axis facilitates liver metastasis of pancreatic ductal adenocarcinoma., PMID:36792623
Targeting Redundant ROBO1 and SDF-1 Pathways Prevents Adult Hemangioblast Derived-EPC and CEC Activity Effectively Blocking Tumor Neovascularization., PMID:36652143
Pan-Cancer Analysis Identifies Tumor Cell Surface Targets for CAR-T Cell Therapies and Antibody Drug Conjugates., PMID:36428765
Novel antigens for targeted radioimmunotherapy in hepatocellular carcinoma., PMID:35708866
Peptide-Based Vaccines for Hepatocellular Carcinoma: A Review of Recent Advances., PMID:34513746
Development of biparatopic bispecific antibody possessing tetravalent scFv-Fc capable of binding to ROBO1 expressed in hepatocellular carcinoma cells., PMID:33844018
teiresias, a Fruitless target gene encoding an immunoglobulin-superfamily transmembrane protein, is required for neuronal feminization in Drosophila., PMID:33087851
Slit2 is a potential biomarker for renal impairment in systemic lupus erythematosus., PMID:33079290
Photosensitizer With Illumination Enhances In Vivo Antitumor Effect of Anti-ROBO1 Immunotoxin on Maxillary Sinus Squamous Cell Carcinoma., PMID:32620618
Saponin Facilitates Anti-Robo1 Immunotoxin Cytotoxic Effects on Maxillary Sinus Squamous Cell Carcinoma., PMID:32256588
Generation of biparatopic antibody through two-step targeting of fragment antibodies on antigen using SpyTag and SpyCatcher., PMID:31993343
Roundabout1 distribution in neoplastic and non-neoplastic diseases with a focus on neoangiogenesis., PMID:31949661
R5, a neutralizing antibody to Robo1, suppresses breast cancer growth and metastasis by inhibiting angiogenesis via down-regulating filamin A., PMID:31811830
Role of angiogenesis in adenomyosis-associated abnormal uterine bleeding and subfertility: a systematic review., PMID:31504506
Affinity Improvement of a Cancer-Targeted Antibody through Alanine-Induced Adjustment of Antigen-Antibody Interface., PMID:30595454
Slit2/Robo4 axis may contribute to endothelial cell dysfunction and angiogenesis disturbance in systemic sclerosis., PMID:30021803
Robo1 Forms a Compact Dimer-of-Dimers Assembly., PMID:29307485
Use of SpyTag/SpyCatcher to construct bispecific antibodies that target two epitopes of a single antigen., PMID:28637250
Slit2‑Robo1 signaling promotes the adhesion, invasion and migration of tongue carcinoma cells via upregulating matrix metalloproteinases 2 and 9, and downregulating E‑cadherin., PMID:27431199
Kinetic exclusion assay of monoclonal antibody affinity to the membrane protein Roundabout 1 displayed on baculovirus., PMID:27095060
Suppression of Slit2/Robo1 mediated HUVEC migration by Robo4., PMID:26713366
Expression of Robo protein in bladder cancer tissues and its effect on the growth of cancer cells by blocking Robo protein., PMID:26617702
Activation of Slit2-Robo1 signaling promotes liver fibrosis., PMID:26264936
90Y-Labeled Anti-ROBO1 Monoclonal Antibody Exhibits Antitumor Activity against Small Cell Lung Cancer Xenografts., PMID:26017283
Motor neuron cell bodies are actively positioned by Slit/Robo repulsion and Netrin/DCC attraction., PMID:25530182
Structural features of interfacial tyrosine residue in ROBO1 fibronectin domain-antibody complex: Crystallographic, thermodynamic, and molecular dynamic analyses., PMID:25492858
A (90)Y-labelled anti-ROBO1 monoclonal antibody exhibits antitumour activity against hepatocellular carcinoma xenografts during ROBO1-targeted radioimmunotherapy., PMID:25006547
Slit2 promotes tumor growth and invasion in chemically induced skin carcinogenesis., PMID:24840330
ROBO1, a tumor suppressor and critical molecular barrier for localized tumor cells to acquire invasive phenotype: study in African-American and Caucasian prostate cancer models., PMID:24752651
New antiangiogenic strategies beyond inhibition of vascular endothelial growth factor with special focus on axon guidance molecules., PMID:24401553
Genome-wide association study of antibody response to Newcastle disease virus in chicken., PMID:23663563
Cerebral endothelial expression of Robo1 affects brain infiltration of polymorphonuclear neutrophils during mouse stroke recovery., PMID:23473743
Slit/Robo1 signaling regulates neural tube development by balancing neuroepithelial cell proliferation and differentiation., PMID:23438940
[Role of Slit-Robo signaling in the proliferation of human mucoepidermoid carcinoma Mc3 cells]., PMID:22366001
Protein markers and differentiation in culture for Schlemm's canal endothelial cells., PMID:22210126
The axonal repellent Slit2 inhibits pericyte migration: potential implications in angiogenesis., PMID:22198087
Effects of Roundabout 5 on adhesion, invasion and potential motility of human tongue carcinoma Tb cells., PMID:21933570
Slit2 regulates attractive eosinophil and repulsive neutrophil chemotaxis through differential srGAP1 expression during lung inflammation., PMID:20944010
Slit-Robo signaling mediates lymphangiogenesis and promotes tumor lymphatic metastasis., PMID:20438712
Effect of Robo1 on retinal pigment epithelial cells and experimental proliferative vitreoretinopathy., PMID:20071679
Targeting Slit-Roundabout signaling inhibits tumor angiogenesis in chemical-induced squamous cell carcinogenesis., PMID:18201275
Slit2 involvement in glioma cell migration is mediated by Robo1 receptor., PMID:17968499
For research use only. Not for human or drug use.
