Catalog No.
PHB52701
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human REC8 (Leu470-His547).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
Cohesin Rec8p, Meiotic recombination protein REC8 homolog, REC8, REC8L1
Purification
Purified by antigen affinity column.
Accession
O95072
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Mapping separase-mediated cleavage in situ., PMID:36415827
Cloning and characterization of rec8 gene in orange-spotted grouper (Epinephelus coioides) and Dmrt1 regulation of rec8 promoter activity., PMID:33547601
REC8 suppresses tumor angiogenesis by inhibition of NF-κB-mediated vascular endothelial growth factor expression in gastric cancer cells., PMID:32958054
Novel components of germline sex determination acting downstream of foxl3 in medaka., PMID:30392839
Cloning and characterization of the homoeologous genes for the Rec8-like meiotic cohesin in polyploid wheat., PMID:30305022
Superresolution microscopy reveals the three-dimensional organization of meiotic chromosome axes in intact Caenorhabditis elegans tissue., PMID:28559338
SUMO-1 plays crucial roles for spindle organization, chromosome congression, and chromosome segregation during mouse oocyte meiotic maturation., PMID:25123474
PAB-1, a Caenorhabditis elegans poly(A)-binding protein, regulates mRNA metabolism in germline by interacting with CGH-1 and CAR-1., PMID:24367695
Overexpression of SETβ, a protein localizing to centromeres, causes precocious separation of chromatids during the first meiosis of mouse oocytes., PMID:23444375
Condensins I and II are essential for construction of bivalent chromosomes in mouse oocytes., PMID:21795393
OsREC8 is essential for chromatid cohesion and metaphase I monopolar orientation in rice meiosis., PMID:21606318
Analysis of oocyte-like cells differentiated from porcine fetal skin-derived stem cells., PMID:21054136
Interlock formation and coiling of meiotic chromosome axes during synapsis., PMID:19752214
Metaphase I arrest in LT/Sv mouse oocytes involves the spindle assembly checkpoint., PMID:18753610
Coupling meiotic chromosome axis integrity to recombination., PMID:18347098
Defective cohesin is associated with age-dependent misaligned chromosomes in oocytes., PMID:18252055
OsRAD21-3, an orthologue of yeast RAD21, is required for pollen development in Oryza sativa., PMID:17617177
Mammalian SGO2 appears at the inner centromere domain and redistributes depending on tension across centromeres during meiosis II and mitosis., PMID:17205076
Loss of Rec8 from chromosome arm and centromere region is required for homologous chromosome separation and sister chromatid separation, respectively, in mammalian meiosis., PMID:16855401
Degradation of securin in mouse and pig oocytes is dependent on ubiquitin-proteasome pathway and is required for proteolysis of the cohesion subunit, Rec8, at the metaphase-to-anaphase transition., PMID:16720305
Changes in the expression and localization of cohesin subunits during meiosis in a non-mammalian vertebrate, the medaka fish., PMID:15261826
Cohesin component dynamics during meiotic prophase I in mammalian oocytes., PMID:15125634
Multi-pathway control of the proliferation versus meiotic development decision in the Caenorhabditis elegans germline., PMID:15063172
Maintenance of cohesin at centromeres after meiosis I in budding yeast requires a kinetochore-associated protein related to MEI-S332., PMID:15062096
Temporally and spatially selective loss of Rec8 protein from meiotic chromosomes during mammalian meiosis., PMID:12759374
Differential immunolocalization of a putative Rec8p in meiotic autosomes and sex chromosomes of triatomine bugs., PMID:12707778
Meiotic cohesin REC8 marks the axial elements of rat synaptonemal complexes before cohesins SMC1beta and SMC3., PMID:12615909
STAG2 and Rad21 mammalian mitotic cohesins are implicated in meiosis., PMID:12034751
A Caenorhabditis elegans cohesion protein with functions in meiotic chromosome pairing and disjunction., PMID:11390355
For research use only. Not for human or drug use.
