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Anti-Human ACO2 Polyclonal Antibody

Catalog #:   PHK43901 Specific References (8) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: Q99798
Overview

Catalog No.

PHK43901

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ACO2 (Glu41-Arg523).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

ACO2, Citrate hydro-lyase, Aconitase, Aconitate hydratase, mitochondrial

Purification

Purified by antigen affinity column.

Accession

Q99798

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with ACO2 antibody (PHK43901) at 1μg/ml.

    Lane 1: Neuro-2a cell lysate
    Lane 2: Jurkat cell lysate
    Lane 3: Mouse brain lysate
    Lane 4: Mouse kidney lysate
    Lane 5: Mouse heart lysate
    Lane 6: Rat brain lysate
    Lane 7: Rat kidney lysate
    Lane 8: Rat heart lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 85 kDa
    Observed MW: 85 kDa
  • Immunohistochemical
    Immunohistochemical analysis of mouse kidney stained for ACO2 with PHK43901.
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with ACO2 antibody (PHK43901) at 1 µg/ml.

    Lane 1: Recombinant Protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 54 kDa
    Observed MW: 54 kDa
References

Curcumin Prevents the Glycation of Tricarboxylic Acid Cycle and Cell Respiration Proteins in the Heart of Mice Fed with a High-fructose Diet., PMID:35362381

Development of a scale-up strategy for Chinese hamster ovary cell culture processes using the kL a ratio as a direct indicator of gas stripping conditions., PMID:32298540

36th International Symposium on Intensive Care and Emergency Medicine : Brussels, Belgium. 15-18 March 2016., PMID:27885969

FoxO3a confers cetuximab resistance in RAS wild-type metastatic colorectal cancer through c-Myc., PMID:27825133

The role of mitochondrial aconitate (ACO2) in human sperm motility., PMID:24785945

ACC oxidase (ACO) genes in Trifolium occidentale (L.) and their relationship to ACO genes in white clover (T. repens L.) and T. pallescens (L.)., PMID:21320784

Characterization of the 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase multigene family of Malus domestica Borkh., PMID:19223050

Production and characterization of monoclonal antibodies specific for Shewanella colwelliana exopolysaccharide., PMID:7686001

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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Distributor list

For research use only. Not for human or drug use.

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Anti-Human ACO2 Polyclonal Antibody [PHK43901]
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