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Anti-Human STX3 Polyclonal Antibody

Catalog #:   PHG46801 Specific References (7) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: Q13277
Overview

Catalog No.

PHG46801

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human STX3 (Ile26-Ile265).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

STX3A, Syntaxin-3, STX3

Purification

Purified by antigen affinity column.

Accession

Q13277

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN-BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with STX3 antibody (PHG46801) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 30 kDa
    Observed MW: 30 kDa
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with STX3 antibody (PHG46801) at 1 μg/ml.

    Lane 1: HepG2
    Lane 2: HEK293T
    Lane 3: BxPC-3
    Lane 4: Raw 264.7
    Lane 5: PC12
    Lane 6: NIH3T3
    Lane 7: Caco-2

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 33 kDa
    Observed MW: 33 kDa
  • Immunohistochemical
    Immunohistochemical analysis of rat kidney tissue stained for STX3 with PHG46801.
References

Tomosyn functions as a PKCδ-regulated fusion clamp in mast cell degranulation., PMID:29970602

Tracking Endocytosis and Intracellular Trafficking of Epitope-tagged Syntaxin 3 by Antibody Feeding in Live, Polarized MDCK Cells., PMID:29564371

Accumulation of non-outer segment proteins in the outer segment underlies photoreceptor degeneration in Bardet-Biedl syndrome., PMID:26216965

Decreased miR-146 expression in peripheral blood mononuclear cells is correlated with ongoing islet autoimmunity in type 1 diabetes patients 1miR-146., PMID:24796653

Syntaxin-4 is implicated in the secretion of antibodies by human plasma cells., PMID:24146186

SNAP-23 and syntaxin-3 are required for chemokine release by mature human mast cells., PMID:21981832

Vesicle associated membrane protein (VAMP)-7 and VAMP-8, but not VAMP-2 or VAMP-3, are required for activation-induced degranulation of mature human mast cells., PMID:18253931

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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Distributor list

For research use only. Not for human or drug use.

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Anti-Human STX3 Polyclonal Antibody [PHG46801]
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