Catalog No.
PHF75701
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human ARF1 (Met1-Lys181).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
ADP-ribosylation factor 1, ARF1
Purification
Purified by antigen affinity column.
Accession
P84077
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
The Arf pathway is required for resolving ER stress during T cell activation., PMID:40405457
Partial Protection of Goats against Haemonchus contortus Achieved with ADP-Ribosylation Factor 1 Encapsulated in PLGA Nanoparticles., PMID:39460354
Expression analysis of type I ARF small GTPases ARF1-3 during mouse brain development., PMID:38227057
Dynamin-like Protein 1 (DNML1) as a Molecular Target for Antibody-Based Immunotherapy to Treat Glaucoma., PMID:36362420
Structural and functional analysis of the small GTPase ARF1 reveals a pivotal role of its GTP-binding domain in controlling of the generation of viral inclusion bodies and replication of grass carp reovirus., PMID:36091067
Cell-matrix adhesion controls Golgi organization and function through Arf1 activation in anchorage-dependent cells., PMID:30054383
Analysis of Membrane Protein Topology in the Plant Secretory Pathway., PMID:28861819
Combined targeting of Arf1 and Ras potentiates anticancer activity for prostate cancer therapeutics., PMID:28830537
Essential role for GABARAP autophagy proteins in interferon-inducible GTPase-mediated host defense., PMID:28604719
Overexpression of ARF1 is associated with cell proliferation and migration through PI3K signal pathway in ovarian cancer., PMID:28098897
GB virus type C E2 protein inhibits human immunodeficiency virus type 1 Gag assembly by downregulating human ADP-ribosylation factor 1., PMID:26675377
Which Way In? The RalF Arf-GEF Orchestrates Rickettsia Host Cell Invasion., PMID:26291822
Hematopoietic stem/progenitor cells directly contribute to arteriosclerotic progression via integrin β2., PMID:25546260
Diverse mechanisms regulate the surface expression of immunotherapeutic target ctla-4., PMID:25538704
Generation of unique poliovirus RNA replication organelles., PMID:24570367
Chemokine unresponsiveness of chronic lymphocytic leukemia cells results from impaired endosomal recycling of Rap1 and is associated with a distinctive type of immunological anergy., PMID:23804711
ARF1-regulated coatomer directs the steady-state localization of protein kinase C epsilon at the Golgi apparatus., PMID:23195223
The Drosophila Arf1 homologue Arf79F is essential for lamellipodium formation., PMID:22992458
ADP ribosylation factor 1 activity is required to recruit AP-1 to the major histocompatibility complex class I (MHC-I) cytoplasmic tail and disrupt MHC-I trafficking in HIV-1-infected primary T cells., PMID:21917951
ARF1 localizes to the golgi and the trans-golgi network., PMID:21406621
Cdc42 regulates microtubule-dependent Golgi positioning., PMID:20525016
Functional characterization of protein-sorting machineries at the trans-Golgi network in Drosophila melanogaster., PMID:20067992
Interaction of brefeldin A-inhibited guanine nucleotide-exchange protein (BIG) 1 and kinesin motor protein KIF21A., PMID:19020088
Proteome analysis of Legionella vacuoles purified by magnetic immunoseparation reveals secretory and endosomal GTPases., PMID:18980612
Association of guanine nucleotide-exchange protein BIG1 in HepG2 cell nuclei with nucleolin, U3 snoRNA, and fibrillarin., PMID:18292223
Rab1b interacts with GBF1 and modulates both ARF1 dynamics and COPI association., PMID:17429068
Confocal microscopy-based linescan methodologies for intra-Golgi localization of proteins., PMID:17341478
AMY-1 (associate of Myc-1) localization to the trans-Golgi network through interacting with BIG2, a guanine-nucleotide exchange factor for ADP-ribosylation factors., PMID:16866877
Mutational analysis of the Arf1*GTP/Arf GAP interface reveals an Arf1 mutant that selectively affects the Arf GAP ASAP1., PMID:16332543
The role of ARF1 and rab GTPases in polarization of the Golgi stack., PMID:16101683
Actin and Arf1-dependent recruitment of a cortactin-dynamin complex to the Golgi regulates post-Golgi transport., PMID:15821732
BIG1 is a binding partner of myosin IXb and regulates its Rho-GTPase activating protein activity., PMID:15644318
LdARF1 in trafficking and structural maintenance of the trans-Golgi cisternal network in the protozoan pathogen Leishmania donovani., PMID:15479452
Differences between AGAP1, ASAP1 and Arf GAP1 in substrate recognition: interaction with the N-terminus of Arf1., PMID:15212764
ARF1 regulates Nef-induced CD4 degradation., PMID:15202998
Nuclear localization and molecular partners of BIG1, a brefeldin A-inhibited guanine nucleotide-exchange protein for ADP-ribosylation factors., PMID:14973189
Membrane-bound fatty acid desaturases are inserted co-translationally into the ER and contain different ER retrieval motifs at their carboxy termini., PMID:14690501
Specific regulation of the adaptor protein complex AP-3 by the Arf GAP AGAP1., PMID:12967569
Identification and localization of a beta-COP-like protein involved in the morphodynamics of the plant Golgi apparatus., PMID:12885863
HIV-1 Nef stabilizes the association of adaptor protein complexes with membranes., PMID:12486136
PGF(2alpha)-induced contraction of cat esophageal and lower esophageal sphincter circular smooth muscle., PMID:12121874
Inhibitors of COP-mediated transport and cholera toxin action inhibit simian virus 40 infection., PMID:12006667
GGA proteins associate with Golgi membranes through interaction between their GGAH domains and ADP-ribosylation factors., PMID:11950392
Regulation and recruitment of phosphatidylinositol 4-kinase on immature secretory granules is independent of ADP-ribosylation factor 1., PMID:11931656
Site-specific cross-linking reveals a differential direct interaction of class 1, 2, and 3 ADP-ribosylation factors with adaptor protein complexes 1 and 3., PMID:11926829
Association of ARF and Rabs with complement receptor type-1 storage vesicles in human neutrophils., PMID:11590205
The mammalian guanine nucleotide exchange factor mSec12 is essential for activation of the Sar1 GTPase directing endoplasmic reticulum export., PMID:11422940
ARF-GEP(100), a guanine nucleotide-exchange protein for ADP-ribosylation factor 6., PMID:11226253
Aux/IAA proteins are phosphorylated by phytochrome in vitro., PMID:11115889
Separation of membrane trafficking and actin remodeling functions of ARF6 with an effector domain mutant., PMID:10913182
For research use only. Not for human or drug use.
