Overview
Catalog No.
PHK42101
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human COQ7 (Ser36-Leu217).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
COQ7, Ubiquinone biosynthesis monooxygenase COQ7, 5-demethoxyubiquinone hydroxylase, mitochondrial, DMQ hydroxylase, Timing protein clk-1 homolog
Purification
Purified by antigen affinity column.
Accession
Q99807
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Data Image
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Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with COQ7 antibody (PHK42101) at 1 μg/ml.
Lane 1: Recombinant protein
Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.
Predict MW: 23 kDa
Observed MW: 23 kDa
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Various lysates were subjected to SDS PAGE followed by western blot with COQ7 antibody (PHK42101) at 1 μg/ml.
Lane 1: Rat heart tissue
Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.
Predict MW: 24 kDa
Observed MW: 24 kDa
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Immunohistochemical analysis of mouse lung tissue stained for COQ7 with PHK42101.
References
Characterization of human mitochondrial PDSS and COQ proteins and their roles in maintaining coenzyme Q10 levels and each other's stability., PMID:32194061
Saposin B is a human coenzyme q10-binding/transfer protein., PMID:18385835
The effect of ubiquinone-7 and its metabolites on the immune response. IV. Chemical structure-adjuvant activity relationship of quinonyl derivatives on humoral immune response., PMID:309457
The effect of ubiquinone-7 and its metabolites on the immune response. II. Adjuvant activity on the circulating antibody production and the effect on the liver lysosomal membrane., PMID:1088635
The effect of ubiquinone-7 and its metabolites on the immune response. III. The effect on the immune response to sheep erythrocytes and DNP-Lys-Ficoll in mice., PMID:1010684
The effect of ubiquinone-7 and its metabolites on the immune response., PMID:4660317
Datasheet