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Anti-Human NONO Polyclonal Antibody (PHH04701)

Host species:Rabbit
Isotype:IgG
Applications:ELISA, IHC, WB
Accession:Q15233
Overview

Catalog No.

PHH04701

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human NONO (Arg73-Arg256).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

54 kDa nuclear RNA- and DNA-binding protein, NRB54, p54nrb, NMT55, NonO protein, p54(nrb), 55 kDa nuclear protein, NONO, DNA-binding p52/p100 complex, 52 kDa subunit, Non-POU domain-containing octamer-binding protein

Purification

Purified by antigen affinity column.

Accession

Q15233

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with NONO antibody (PHH04701) at 1μg/ml.

    Lane 1: NIH3T3 cell lysate
    Lane 2: PC12 cell lysate
    Lane 3: 293T cell lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 54 kDa
    Observed MW: 54 kDa
  • Immunohistochemical
    Immunohistochemical analysis of mouse brain stained for NONO with PHH04701.
References
Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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Distributor list

For research use only. Not for human or drug use.

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Anti-Human NONO Polyclonal Antibody [PHH04701]
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