Catalog No.
PHB60301
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human VAPB (Met1-Glu124).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
VAMP-B/VAMP-C, VAPB, VAP-B/VAP-C, Vesicle-associated membrane protein-associated protein B/C, VAMP-associated protein B/C
Purification
Purified by antigen affinity column.
Accession
O95292
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Novel extracellular vesicle release pathway facilitated by toxic superoxide dismutase 1 oligomers., PMID:40291716
Understanding the physiological role and cross-interaction network of VapBC35 toxin-antitoxin system from Mycobacterium tuberculosis., PMID:40016306
A guide to selecting high-performing antibodies for VAPB (UniProt ID: O95292) for use in western blot, immunoprecipitation, and immunofluorescence., PMID:39916983
Shared mechanisms of enhanced plasmid maintenance and antibiotic tolerance mediated by the VapBC toxin:antitoxin system., PMID:39704502
Deciphering the role of VapBC toxin-antitoxin systems in Mycobacterium tuberculosis stress adaptation., PMID:39431307
Phosphorylation of VapB antitoxins affects intermolecular interactions to regulate VapC toxin activity in Mycobacterium tuberculosis., PMID:39315797
Stay or Go: Sulfolobales Biofilm Dispersal Is Dependent on a Bifunctional VapB Antitoxin., PMID:37036347
Assessing Peroxisomal Protein Interaction by Immunoprecipitation., PMID:36952197
Apoptosis like symptoms associated with abortive infection of Mycobacterium smegmatis by mycobacteriophage D29., PMID:35580120
tRNAfMet Inactivating Mycobacterium tuberculosis VapBC Toxin-Antitoxin Systems as Therapeutic Targets., PMID:35404073
Role of Vesicle-Associated Membrane Protein-Associated Proteins (VAP) A and VAPB in Nuclear Egress of the Alphaherpesvirus Pseudorabies Virus., PMID:34200728
Role of VAPB and vesicular profiles in α-synuclein aggregates in multiple system atrophy., PMID:34196429
Structural and Functional Study of the Klebsiella pneumoniae VapBC Toxin-Antitoxin System, Including the Development of an Inhibitor That Activates VapC., PMID:33146528
Genome-wide interaction screen for Mycobacterium tuberculosis ClpCP protease reveals toxin-antitoxin systems as a major substrate class., PMID:32301575
Misfolded SOD1 inclusions in patients with mutations in C9orf72 and other ALS/FTD-associated genes., PMID:30992335
Immunotherapeutic effects of Mycobacterium tuberculosis rv3407 DNA vaccine in mice., PMID:30632804
Structural characterization of VapB46 antitoxin from Mycobacterium tuberculosis: insights into VapB46-DNA binding., PMID:30576065
Induction of a Toxin-Antitoxin Gene Cassette under High Hydrostatic Pressure Enables Markerless Gene Disruption in the Hyperthermophilic Archaeon Pyrococcus yayanosii., PMID:30504216
VapC proteins from Mycobacterium tuberculosis share ribonuclease sequence specificity but differ in regulation and toxicity., PMID:30169502
Crystal structure of Mycobacterium tuberculosis VapC20 toxin and its interactions with cognate antitoxin, VapB20, suggest a model for toxin-antitoxin assembly., PMID:28986943
Structural Determinants for Antitoxin Identity and Insulation of Cross Talk between Homologous Toxin-Antitoxin Systems., PMID:27672196
Characterization of the Deep-Sea Streptomyces sp. SCSIO 02999 Derived VapC/VapB Toxin-Antitoxin System in Escherichia coli., PMID:27376329
The VapBC1 toxin-antitoxin complex from Mycobacterium tuberculosis: purification, crystallization and X-ray diffraction analysis., PMID:27303903
Structure-function analysis of VapB4 antitoxin identifies critical features of a minimal VapC4 toxin-binding module., PMID:25622615
Crystal structure of the VapBC-15 complex from Mycobacterium tuberculosis reveals a two-metal ion dependent PIN-domain ribonuclease and a variable mode of toxin-antitoxin assembly., PMID:25450593
Analysis of non-typeable Haemophilous influenzae VapC1 mutations reveals structural features required for toxicity and flexibility in the active site., PMID:25391136
VapC from the leptospiral VapBC toxin-antitoxin module displays ribonuclease activity on the initiator tRNA., PMID:25047537
Toxin-antitoxin vapBC locus participates in formation of the dormant state in Mycobacterium smegmatis., PMID:24417293
Expression of vesicle-associated membrane-protein-associated protein B cleavage products in peripheral blood leukocytes and cerebrospinal fluid of patients with sporadic amyotrophic lateral sclerosis., PMID:24372953
Preliminary crystallographic analysis of recombinant VapBC-15 toxin-antitoxin complex from Mycobacterium tuberculosis., PMID:24192359
Morphological and biochemical characterization of the membranous hepatitis C virus replication compartment., PMID:23885072
Regulation of the vapBC-1 toxin-antitoxin locus in nontypeable Haemophilus influenzae., PMID:22427824
A VapBC toxin-antitoxin module is a posttranscriptional regulator of metabolic flux in mycobacteria., PMID:22366418
VapC toxins from Mycobacterium tuberculosis are ribonucleases that differentially inhibit growth and are neutralized by cognate VapB antitoxins., PMID:21738782
Novel splice variants of the amyotrophic lateral sclerosis-associated gene VAPB expressed in human tissues., PMID:20227395
Characterization of monoclonal antibodies against the nonstructural 5A protein of hepatitis C virus., PMID:19396577
Human VAP-B is involved in hepatitis C virus replication through interaction with NS5A and NS5B., PMID:16227268
Virulence plasmid of Rhodococcus equi contains inducible gene family encoding secreted proteins., PMID:11159951
Molecular characterization of a genomic region associated with virulence in Dichelobacter nodosus., PMID:1398971
For research use only. Not for human or drug use.
