Anti-Human RPTOR Polyclonal Antibody

Catalog No.

PHK40101

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human RPTOR (Arg1012-Arg1335).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

KIAA1303, Raptor, RAPTOR, RPTOR, Regulatory-associated protein of mTOR, p150 target of rapamycin (TOR)-scaffold protein

Purification

Purified by antigen affinity column.

Accession

Q8N122

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Genetic and pharmacological targeting of mTORC1 in mouse models of arteriovenous malformation expose non-cell autonomous signalling in HHT., PMID:39661206

Longitudinal plasma proteome profiling reveals the diversity of biomarkers for diagnosis and cetuximab therapy response of colorectal cancer., PMID:38302471

Rngtt governs biliary-derived liver regeneration initiation by transcriptional regulation of mTORC1 and Dnmt1 in zebrafish., PMID:36724876

Effect of resistance training on satellite cells in old mice - a transcriptome study : implications for sarcopenia., PMID:35188421

Cardamonin inhibits cell proliferation by caspase-mediated cleavage of Raptor., PMID:33043385

mTORC1 coordinates an immediate unfolded protein response-related transcriptome in activated B cells preceding antibody secretion., PMID:32024827

Raptor directs Sertoli cell cytoskeletal organization and polarity in the mouse testis., PMID:29961810

A mechanistic target of rapamycin complex 1/2 (mTORC1)/V-Akt murine thymoma viral oncogene homolog 1 (AKT1)/cathepsin H axis controls filaggrin expression and processing in skin, a novel mechanism for skin barrier disruption in patients with atopic dermatitis., PMID:27913303

mTOR has distinct functions in generating versus sustaining humoral immunity., PMID:27760048

Down-regulation of phosphoglucose isomerase/autocrine motility factor enhances gensenoside Rh2 pharmacological action on leukemia KG1α cells., PMID:24606425

Disruption of an hTERT-mTOR-RAPTOR protein complex by a phytochemical perillyl alcohol and rapamycin., PMID:23283642

Intestinal cell kinase (ICK) promotes activation of mTOR complex 1 (mTORC1) through phosphorylation of Raptor Thr-908., PMID:22356909

Placental DEPTOR as a stress sensor during pregnancy., PMID:21992080

Raptor, a positive regulatory subunit of mTOR complex 1, is a novel phosphoprotein of the rDNA transcription machinery in nucleoli and chromosomal nucleolus organizer regions (NORs)., PMID:21900751

mTOR kinase domain phosphorylation promotes mTORC1 signaling, cell growth, and cell cycle progression., PMID:21576368

ULK1 inhibits mTORC1 signaling, promotes multisite Raptor phosphorylation and hinders substrate binding., PMID:21460630

ERK1/2 phosphorylate Raptor to promote Ras-dependent activation of mTOR complex 1 (mTORC1)., PMID:21071439

Steady-state kinetic and inhibition studies of the mammalian target of rapamycin (mTOR) kinase domain and mTOR complexes., PMID:20804212

Chronic inhibition of the mTORC1/S6K1 pathway increases insulin-induced PI3K activity but inhibits Akt2 and glucose transport stimulation in 3T3-L1 adipocytes., PMID:20203102

Raptor is phosphorylated by cdc2 during mitosis., PMID:20169205

Regulation of mTOR complex 1 (mTORC1) by raptor Ser863 and multisite phosphorylation., PMID:19864431

Specific activation of mTORC1 by Rheb G-protein in vitro involves enhanced recruitment of its substrate protein., PMID:19299511

Activation of mammalian target of rapamycin (mTOR) by insulin is associated with stimulation of 4EBP1 binding to dimeric mTOR complex 1., PMID:16798736

Suppression of the mTOR-raptor signaling pathway by the inhibitor of heat shock protein 90 geldanamycin., PMID:16428328

Rictor, a novel binding partner of mTOR, defines a rapamycin-insensitive and raptor-independent pathway that regulates the cytoskeleton., PMID:15268862

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