Overview
Catalog No.
PHK40001
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human MKS1 (Gly309-Ser502).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
MKS1, Meckel syndrome type 1 protein
Purification
Purified by antigen affinity column.
Accession
Q9NXB0
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Data Image
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Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with MKS1 antibody (PHK40001) at 1 μg/ml.
Lane 1: Recombinant protein
Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.
Predict MW: 24 kDa
Observed MW: 24 kDa
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Various lysates were subjected to SDS PAGE followed by western blot with MKS1 antibody (PHK40001) at 1 μg/ml.
Lane 1: Hela
Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.
Predict MW: 64 kDa
Observed MW: 64 kDa
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Immunohistochemical analysis of human kidney stained for MKS1 with PHK40001.
References
An alternative model for the role of RP2 protein in flagellum assembly in the African trypanosome., PMID:24257747
Aberrant Wnt signalling and cellular over-proliferation in a novel mouse model of Meckel-Gruber syndrome., PMID:23454480
Renal cystic disease proteins play critical roles in the organization of the olfactory epithelium., PMID:21614130
Nesprin-2 interacts with meckelin and mediates ciliogenesis via remodelling of the actin cytoskeleton., PMID:19596800
Molecular architecture of the centriole proteome: the conserved WD40 domain protein POC1 is required for centriole duplication and length control., PMID:19109428
Datasheet
For research use only. Not for human or drug use.
