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Anti-Human FXYD1 Polyclonal Antibody

Catalog #:   PHA03801 Specific References (8) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: O00168
Overview

Catalog No.

PHA03801

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human FXYD1 (Glu21-Arg92).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Sodium/potassium-transporting ATPase subunit FXYD1, PLM, FXYD1, Phospholemman, FXYD domain-containing ion transport regulator 1

Purification

Purified by antigen affinity column.

Accession

O00168

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN-BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with FXYD1 antibody (PHA03801) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 37 kDa
    Observed MW: 37 kDa
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with FXYD1 antibody (PHA03801) at 1 μg/ml.

    Lane 1: Rat liver
    Lane 2: Rat cardiac muscle
    Lane 3: Mouse cardiac muscle
    Lane 4: HepG2
    Lane 5: Hela

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 10 kDa
    Observed MW: 10 kDa
  • Immunohistochemical
    Immunohistochemical analysis of mouse heart stained for FXYD1 with PHA03801.
References

Abnormal Scn1b and Fxyd1 gene expression in the pulled-through ganglionic colon may influence functional outcome in patients with Hirschsprung's disease., PMID:30386899

Importance of the Voltage Dependence of Cardiac Na/K ATPase Isozymes., PMID:26536262

Oxidative inhibition of the vascular Na+-K+ pump via NADPH oxidase-dependent β1-subunit glutathionylation: implications for angiotensin II-induced vascular dysfunction., PMID:23816524

FXYD proteins reverse inhibition of the Na+-K+ pump mediated by glutathionylation of its beta1 subunit., PMID:21454534

Exercise-induced regulation of phospholemman (FXYD1) in rat skeletal muscle: implications for Na+/K+-ATPase activity., PMID:18373741

Functional interactions of phospholemman (PLM) (FXYD1) with Na+,K+-ATPase. Purification of alpha1/beta1/PLM complexes expressed in Pichia pastoris., PMID:16608841

Phosphohippolin expression in the rat central nervous system., PMID:15193427

Phospholemman, a single-span membrane protein, is an accessory protein of Na,K-ATPase in cerebellum and choroid plexus., PMID:12657675

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-Human FXYD1 Polyclonal Antibody [PHA03801]
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