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Anti-Human PPAT Polyclonal Antibody

Catalog #:   PHG08001 Specific References (24) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: Q06203
Overview

Catalog No.

PHG08001

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PPAT (Glu11-Lys474).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

ATase, Amidophosphoribosyltransferase, PPAT, GPAT, Glutamine phosphoribosylpyrophosphate amidotransferase

Purification

Purified by antigen affinity column.

Accession

Q06203

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN-BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with PPAT antibody (PHG08001) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 54 kDa
    Observed MW: 54 kDa
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with PPAT antibody (PHG08001) at 1 μg/ml.

    Lane 1: HepG2
    Lane 2: HCT116

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 57 kDa
    Observed MW: 57 kDa
  • Immunohistochemical
    Immunohistochemical analysis of Human cervical cancer tissue stained for PPAT with PHG08001.
References

Correction: Single-cell transcriptome profiling and the use of AID deficient mice reveal that B cell activation combined with antibody class switch recombination and somatic hypermutation do not benefit the control of experimental trypanosomosis., PMID:39058654

Correction: Understanding antibody-dependent enhancement in dengue: Are afucosylated IgG1s a concern?, PMID:37851611

Correction: A vesicular stomatitis virus-based prime-boost vaccination strategy induces potent and protective neutralizing antibodies against SARS-CoV-2., PMID:36445868

Correction: Current status of therapeutic monoclonal antibodies against SARS-CoV-2., PMID:36409672

Correction: Anti-prothrombin autoantibodies enriched after infection with SARS-CoV-2 and influenced by strength of antibody response against SARS-CoV-2 proteins., PMID:35108341

Correction: Breadth and function of antibody response to acute SARS-CoV-2 infection in humans., PMID:34890427

Correction: Protective porcine influenza virus-specific monoclonal antibodies recognize similar haemagglutinin epitopes as humans., PMID:34347851

Correction: RV144 HIV-1 vaccination impacts post-infection antibody responses., PMID:33651828

Correction: Broad dengue neutralization in mosquitoes expressing an engineered antibody., PMID:32348355

Correction: Neutralization-guided design of HIV-1 envelope trimers with high affinity for the unmutated common ancestor of CH235 lineage CD4bs broadly neutralizing antibodies., PMID:31790514

Dissecting the Role of DDX21 in Regulating Human Cytomegalovirus Replication., PMID:31554690

Correction: HIV-1 envelope glycan modifications that permit neutralization by germline-reverted VRC01-class broadly neutralizing antibodies., PMID:30913265

Correction: Influenza A virus hemagglutinin glycosylation compensates for antibody escape fitness costs., PMID:29924863

Correction: Features of Recently Transmitted HIV-1 Clade C Viruses that Impact Antibody Recognition: Implications for Active and Passive Immunization., PMID:28945784

Correction: Naturally Acquired Human Immunity to Pneumococcus Is Dependent on Antibody to Protein Antigens., PMID:28273179

Correction: Minimally Mutated HIV-1 Broadly Neutralizing Antibodies to Guide Reductionist Vaccine Design., PMID:27627763

Correction: Antigen-Specific Antibody Glycosylation Is Regulated via Vaccination., PMID:27253391

Correction: Targeted Isolation of Antibodies Directed against Major Sites of SIV Env Vulnerability., PMID:27219002

Correction: Prion infections and anti-PrP antibodies trigger converging neurotoxic pathways., PMID:25875479

A virus-like particle system identifies the endonuclease domain of Crimean-Congo hemorrhagic fever virus., PMID:25810550

The Kaposi's sarcoma-associated herpesvirus (KSHV)-induced 5-lipoxygenase-leukotriene B4 cascade plays key roles in KSHV latency, monocyte recruitment, and lipogenesis., PMID:24335295

Anti-glutamate receptor ɛ2 antibodies in psychiatric patients with anti-thyroid autoantibodies--a prevalence study in Japan., PMID:23142717

A Patient with Postpartum Hypopituitarism (Sheehan's Syndrome) Developed Postpartum Autoimmune Thyroiditis (Transient Thyrotoxicosis and Hypothyroidism): A Case Report and Review of the Literature., PMID:21603170

Thailand's head-on approach., PMID:12315984

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-Human PPAT Polyclonal Antibody [PHG08001]
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