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Anti-Human CUL4A Polyclonal Antibody

Catalog #:   PHG61501 Specific References (11) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: Q13619
Overview

Catalog No.

PHG61501

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CUL4A (Arg666-Ala759).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

CUL-4A, CUL4A, Cullin-4A

Purification

Purified by antigen affinity column.

Accession

Q13619

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with CUL4A antibody (PHG61501) at 1μg/ml.

    Lane 1: PC-12 cell lysate
    Lane 2: Raji cell lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 88 kDa
    Observed MW: 88 kDa
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with CUL4A antibody (PHG61501) at 1 µg/ml.

    Lane 1: NIH3T3 cell lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 88 kDa
    Observed MW: 88 kDa
  • Immunohistochemical
    Immunohistochemical analysis of rat heart stained for CUL4A with PHG61501.
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with CUL4A antibody (PHG61501) at 1 µg/ml.

    Lane 1: Recombinant Protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 25 kDa
    Observed MW: 25 kDa
References

Enhanced Production of HCV E1E2 Subunit Vaccine Candidates via Protein-Protein Interaction Identification in Glycoengineered CHO cells., PMID:40475571

CUL4A-mediated ZEB1/microRNA-340-5p/HMGB1 axis promotes the development of osteoporosis., PMID:37253097

Ddb1 Is Essential for the Expansion of CD4+ Helper T Cells by Regulating Cell Cycle Progression and Cell Death., PMID:34526995

Impact of HIV-1 Vpr manipulation of the DNA repair enzyme UNG2 on B lymphocyte class switch recombination., PMID:32778120

Aiolos collaborates with Blimp-1 to regulate the survival of multiple myeloma cells., PMID:26823144

CENP-A K124 Ubiquitylation Is Required for CENP-A Deposition at the Centromere., PMID:25727006

Involvement of the single Cul4 gene of Chinese mitten crab Eriocheir sinensis in spermatogenesis., PMID:24334119

Modulation of NKG2D-mediated cytotoxic functions of natural killer cells by viral protein R from HIV-1 primary isolates., PMID:21957298

Comprehensive characterization of the DNA amplification at 13q34 in human breast cancer reveals TFDP1 and CUL4A as likely candidate target genes., PMID:19995430

L2DTL/CDT2 interacts with the CUL4/DDB1 complex and PCNA and regulates CDT1 proteolysis in response to DNA damage., PMID:16861906

Paramyxoviruses SV5 and HPIV2 assemble STAT protein ubiquitin ligase complexes from cellular components., PMID:12504558

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

Contact Information

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For research use only. Not for human or drug use.

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Anti-Human CUL4A Polyclonal Antibody [PHG61501]
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