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Anti-Human SIRT5 Polyclonal Antibody

Catalog #:   PHK20701 Specific References (12) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: Q9NXA8
Overview

Catalog No.

PHK20701

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human SIRT5 (Met40-Ser310).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

SIR2-like protein 5, SIRT5, SIR2L5, Regulatory protein SIR2 homolog 5, NAD-dependent protein deacylase sirtuin-5, mitochondrial

Purification

Purified by antigen affinity column.

Accession

Q9NXA8

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN-BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with SIRT5 antibody (PHK20701) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 32 kDa
    Observed MW: 32 kDa
References

The Clinical Role of SIRT-3 in the Acute Rejection Process of Kidney Transplantation and Its Effects on Graft Outcomes: Evaluation of Biomarker Potential., PMID:40142268

Possible pathogenic mechanisms for doxorubicin-induced splenic atrophy in a human breast cancer xenograft mouse model., PMID:38943348

Exploring the role of mitochondrial proteins SIRT5 and MRPL33 through Mendelian randomization in primary biliary cholangitis., PMID:38857754

Inhibition of integrin receptors reduces extracellular matrix levels, ameliorating benign prostate hyperplasia., PMID:37659484

In-depth analysis of the Sirtuin 5-regulated mouse brain malonylome and succinylome using library-free data-independent acquisitions., PMID:36479818

Protein succinylation associated with the progress of hepatocellular carcinoma., PMID:36308411

Sirtuin 5 depletion impairs mitochondrial function in human proximal tubular epithelial cells., PMID:34330933

Targeting a Sirt5-Positive Subpopulation Overcomes Multidrug Resistance in Wild-Type Kras Colorectal Carcinomas., PMID:29514096

Ethanol metabolism modifies hepatic protein acylation in mice., PMID:24073283

The first identification of lysine malonylation substrates and its regulatory enzyme., PMID:21908771

SIRT1 and SIRT5 activity expression and behavioral responses to calorie restriction., PMID:21826711

Mechanism-based affinity capture of sirtuins., PMID:21184005

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

Contact Information

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Distributor list

For research use only. Not for human or drug use.

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Anti-Human SIRT5 Polyclonal Antibody [PHK20701]
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