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Anti-Human H4C1 Polyclonal Antibody (PHF53201)

Host species:Rabbit
Isotype:IgG
Applications:ELISA, IHC, WB
Accession:P62805
Overview

Catalog No.

PHF53201

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human H4C1 (Asn26-Gly103).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

HIST1H4D, HIST2H4A, H4FE, H4FO, H4/D, H4/A, HIST1H4E, HIST1H4F, H4FG, HIST1H4L, H4FD, H4/H, HIST1H4B, HIST1H4I, H4FN, HIST1H4C, H4FI, H4FC, H4/C, H4/E, Histone H4, H4/G, H4/O, H4FB, H4FK, H4/M, H4/B, H4C1, HIST2H4, H4F2, HIST2H4B, HIST1H4A, HIST1H4J, H4/N, H4FH, H4/I, H4/J, H4/K, H4FJ, HIST1H4H, HIST1H4K, HIST4H4, H4FM, H4FA

Purification

Purified by antigen affinity column.

Accession

P62805

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN-BLOT
    Various lysates were subjected to SDS PAGE followed by western blot with H4C1 antibody (PHF53201) at 1 µg/ml.

    Lane 1: Hela cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: MCF-7 cell lysate
    Lane 4: 293T cell lysate
    Lane 5: HL-60 cell lysate
    Lane 6: Raji cell lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 11 kDa
    Observed MW: 13 kDa
  • WESTERN-BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with H4C1 antibody (PHF53201) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 22 kDa
    Observed MW: 22 kDa
References
Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

Contact Information

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Distributor list

For research use only. Not for human or drug use.

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Anti-Human H4C1 Polyclonal Antibody [PHF53201]
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