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Anti-Human IFT20 Polyclonal Antibody

Catalog #:   PHK36001 Specific References (10) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: Q8IY31
Overview

Catalog No.

PHK36001

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human IFT20 (Met1-Lys132).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

hIFT20, IFT20, Intraflagellar transport protein 20 homolog

Purification

Purified by antigen affinity column.

Accession

Q8IY31

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN-BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with IFT20 antibody (PHK36001) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 17 kDa
    Observed MW: 17 kDa
References

Regulation of c-SMAC formation and AKT-mTOR signaling by the TSG101-IFT20 axis in CD4+ T cells., PMID:37029318

Identification of a new aggressive axis driven by ciliogenesis and absence of VDAC1-ΔC in clear cell Renal Cell Carcinoma patients., PMID:32194829

SPAG17 Is Required for Male Germ Cell Differentiation and Fertility., PMID:29690537

Fixation methods can differentially affect ciliary protein immunolabeling., PMID:28352462

The MAL protein is crucial for proper membrane condensation at the ciliary base, which is required for primary cilium elongation., PMID:25967552

Primary cilia in rat mature Müller glia: downregulation of IFT20 expression reduces sonic hedgehog-mediated proliferation and dedifferentiation potential of Müller glia primary cultures., PMID:25504432

Cluap1 is essential for ciliogenesis and photoreceptor maintenance in the vertebrate eye., PMID:24970261

Trichoplein and Aurora A block aberrant primary cilia assembly in proliferating cells., PMID:22529102

Pericentrin forms a complex with intraflagellar transport proteins and polycystin-2 and is required for primary cilia assembly., PMID:15337773

IFT20 links kinesin II with a mammalian intraflagellar transport complex that is conserved in motile flagella and sensory cilia., PMID:12821668

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

Contact Information

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Distributor list

For research use only. Not for human or drug use.

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Anti-Human IFT20 Polyclonal Antibody [PHK36001]
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