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Anti-Human TOMM20 Polyclonal Antibody

Catalog #:   PHH08101 Specific References (27) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: Q15388
Overview

Catalog No.

PHH08101

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human TOMM20 (Arg51-Glu145).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Mitochondrial 20 kDa outer membrane protein, Mitochondrial import receptor subunit TOM20 homolog, KIAA0016, TOMM20, Outer mitochondrial membrane receptor Tom20

Purification

Purified by antigen affinity column.

Accession

Q15388

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with TOMM20 antibody (PHH08101) at 1µg/ml.

    Lane 1: Hela cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: Jurkat cell lysate
    Lane 4: THP-1 cell lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 16 kDa
    Observed MW: 16 kDa
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with TOMM20 antibody (PHH08101) at 1 µg/ml.

    Lane 1: Recombinant Protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 39 kDa
    Observed MW: 39 kDa
References

Inactivation of SIAH-1 E3 ligase attenuates Aβ toxicity by suppressing ubiquitin-dependent DVE-1 degradation in Caenorhabditis elegans models of Alzheimer's disease., PMID:40349774

NLRP3 inflammasome inhibits mitophagy during the progression of temporal lobe epilepsy., PMID:40348802

Senecavirus A induces mitophagy to promote self-replication through direct interaction of 2C protein with K27-linked ubiquitinated TUFM catalyzed by RNF185., PMID:38084826

Tissue imaging reveals disruption of epithelial mitochondrial networks and loss of mitochondria-associated cytochrome-C in inflamed human and murine colon., PMID:36356719

Fibroblast-epithelial metabolic coupling in laryngeal cancer., PMID:36327815

Lens Aquaporin-5 Inserts Into Bovine Fiber Cell Plasma Membranes Via Unconventional Protein Secretion., PMID:35816045

Generation of an Ihh-mKate2-Dre knock-in mouse line., PMID:35765931

Flow Cytometric Analysis of Multiple Mitochondrial Parameters in Human Induced Pluripotent Stem Cells and Their Neural and Glial Derivatives., PMID:34806709

BNIP3L-mediated mitophagy is required for mitochondrial remodeling during the differentiation of optic nerve oligodendrocytes., PMID:33404293

Raft-like lipid microdomains drive autophagy initiation via AMBRA1-ERLIN1 molecular association within MAMs., PMID:33034545

The AMPK-MFN2 axis regulates MAM dynamics and autophagy induced by energy stresses., PMID:32249716

Increased number of mitochondria in capillaries distributed in stroke-like lesions of two patients with MELAS., PMID:31408922

Post-embedding Mammalian Tissue for Immunoelectron Microscopy: A Standardized Procedure Based on Heat-Induced Antigen Retrieval., PMID:27515088

Engineered Upconversion Nanoparticles for Resolving Protein Interactions inside Living Cells., PMID:27510808

Gsk3β and Tomm20 are substrates of the SCFFbxo7/PARK15 ubiquitin ligase associated with Parkinson's disease., PMID:27503909

Spatial profiles of markers of glycolysis, mitochondria, and proton pumps in a rat glioma suggest coordinated programming for proliferation., PMID:26032618

A reverse Warburg metabolism in oral squamous cell carcinoma is not dependent upon myofibroblasts., PMID:25420473

Immunocytochemical localization of the translocase of the outer mitochondrial membrane (Tom20) in the human cochlea., PMID:23165776

Interaction between the human mitochondrial import receptors Tom20 and Tom70 in vitro suggests a chaperone displacement mechanism., PMID:21771790

TOM22, a core component of the mitochondria outer membrane protein translocation pore, is a mitochondrial receptor for the proapoptotic protein Bax., PMID:17096026

Voltage-dependent anion channel localises to the plasma membrane and peripheral but not perinuclear mitochondria., PMID:12698369

Tom34 unlike Tom20 does not interact with the leader sequences of mitochondrial precursor proteins., PMID:11913975

Characterization of the novel mitochondrial protein import component, Tom34, in mammalian cells., PMID:10101285

Characterization of the initial steps of precursor import into rat liver mitoplasts., PMID:9756630

Interactions of the human mitochondrial protein import receptor, hTom20, with precursor proteins in vitro reveal pleiotropic specificities and different receptor domain requirements., PMID:9211931

A receptor for the import of proteins into human mitochondria., PMID:8654425

Identification of the human mitochondrial protein import receptor, huMas20p. Complementation of delta mas20 in yeast., PMID:7589431

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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Anti-Human TOMM20 Polyclonal Antibody [PHH08101]
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